Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives

Journal of Chromatography. B, Biomedical Sciences and Applications
A K BattaD Alberts

Abstract

Gas chromatographic separations of n-butyl ester-trimethylsilyl ether derivatives of several common bile acids were compared with those of the corresponding methyl ester-trimethylsilyl ether derivatives on a CP-Sil-5 CB capillary column. Both types of derivatives were similarly resolved from each other. However, the n-butyl ester-trimethylsilyl ether derivatives of the bile acids showed longer retention times than the corresponding methyl ester-trimethylsilyl ethers and unlike the methyl ester-trimethylsilyl ether derivatives, were completely resolved from and eluted later than the trimethylsilyl ethers of common plasma sterols including sitosterol. A simplified method of plasma work-up for quantitation of bile acids and application of the above method in quantification of plasma bile acids in humans is described.

Citations

Jul 13, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ashok K BattaDavid L Earnest
Jul 16, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Andrés CriadoMiguel Valcárcel
Dec 17, 2009·Journal of Lipid Research·William J Griffiths, Jan Sjövall
Aug 27, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·David MassonMahfoud Assem
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi
Mar 18, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A K Batta, G Salen

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