PMID: 8586063Oct 1, 1995Paper

Capillary gel electrophoresis separation of high-mannose type oligosaccharides derivatized by 1-aminopyrene-3,6,8-trisulfonic acid

Electrophoresis
A Guttman, T Pritchett

Abstract

In this paper we report the capillary gel electrophoresis separation of 1-aminopyrene-3,6,8-trisulfonic acid (APTS) labeled oligosaccharides, released enzymatically from bovine pancreatic ribonuclease B. The released and labeled high-mannose structures were identified by spiking the separated peaks with the appropriate commercially available individual oligosaccharides. Baseline separation of the three positional isomers of the mannose-7 and mannose-8 oligosaccharides was attained. Comparison of the electrophoretic mobilities of the high-mannose type branched carbohydrates to the linear molecules of maltooligosaccharides (glucose oligomers) have been shown using different gel concentrations in the running buffer system. We observed that increasing gel concentration in the running buffer causes an increase in the relative mobility values of the high-mannose type carbohydrate molecules compared to the linear glucose oligomers. Analysis of our data indicated that this increase in relative migration time was not due to sieving, but seemed to be related to the mannose content and hydrodynamic volume of the branched glycans as well as to the viscosity of the separation medium.

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Citations

Feb 1, 1996·Electrophoresis·Z El Rassi, Y Mechref
Mar 13, 2010·Analytical and Bioanalytical Chemistry·L R RuhaakM Wuhrer
Oct 12, 2012·Journal of the American Society for Mass Spectrometry·Feifei ZhuDavid E Clemmer
Aug 28, 2007·Journal of the American Society for Mass Spectrometry·Catherine E CostelloJohn F Cipollo
Feb 3, 2009·Journal of the American Society for Mass Spectrometry·Justin M PrienVernon N Reinhold
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Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fotini N LamariNikos K Karamanos
Feb 23, 2010·Analytical Chemistry·Zoltan SzaboBarry L Karger
Apr 9, 2013·Analytical Chemistry·Stefan MittermayrAndrás Guttman
Feb 14, 2002·Chemical Reviews·Yehia Mechref, Milos V Novotny
Apr 5, 2007·Nature Protocols·Wouter LaroyNico Callewaert
Feb 5, 2010·Biological Chemistry·Dieter VanderschaegheNico Callewaert
May 3, 2014·Analytical and Bioanalytical Chemistry·Mercedes CasadoRafael Artuch
Jan 18, 2013·Molecular & Cellular Proteomics : MCP·Yehia MechrefHaixu Tang
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Dec 18, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigeo Suzuki

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