Capillary liquid chromatography at ultrahigh pressures

Annual Review of Analytical Chemistry
J W Jorgenson

Abstract

Ultrahigh-pressure liquid chromatography (UHPLC) is a method of liquid chromatography utilizing sub-2-microm particles packed into capillary columns 25 to 100 cm long. Columns of this length packed with particles this fine require operation with pressures from 1,000 to 7,000 bar (15,000 to 100,000 psi). The advantages of this technique are high separation powers (theoretical plate counts from 100,000 to 300,000) and run times from a few minutes (isocratic) to a few hours (long gradients). This review discusses the background and theoretical basis of UHPLC, practical aspects of UHPLC hardware, examples of separations, future areas for research in UHPLC, and techniques that are both competitive with and complementary to UHPLC.

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Citations

Apr 6, 2012·Journal of the American Society for Mass Spectrometry·Roxana E Iacob, John R Engen
Aug 14, 2012·Analytical Chemistry·Debolina ChatterjeeAdam T Woolley
Dec 19, 2012·Bioanalysis·James W HowardColin S Creaser
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Nov 26, 2015·Analytical Chemistry·Jelle De VosSebastiaan Eeltink
Sep 20, 2011·Journal of Separation Science·Matthias Verstraeten, Gert Desmet
Jun 7, 2015·Annual Review of Analytical Chemistry·John R Engen, Thomas E Wales
Oct 11, 2011·Biochimica Et Biophysica Acta·Maureen A Kane
Dec 3, 2014·Journal of Chromatography. a·James P GriniasJames W Jorgenson
Feb 15, 2014·Journal of Analytical Methods in Chemistry·Alan S ChanYonggang Zhu
Feb 28, 2017·Analytical Chemistry·Josef J HeilandDetlev Belder
Oct 31, 2018·Molecular & Cellular Proteomics : MCP·Sergey I KovalchukAdelina Rogowska-Wrzesinska
Jan 22, 2020·Journal of Separation Science·Samuel W FosterJames P Grinias
Dec 16, 2010·Rapid Communications in Mass Spectrometry : RCM·Alicia L RichardsSarah Trimpin
Feb 22, 2011·Rapid Communications in Mass Spectrometry : RCM·Mehdi MoiniSamir Bootwala
Sep 10, 2020·Analytical Methods : Advancing Methods and Applications·Katherine L Sanders, James L Edwards
Feb 1, 2019·Molecular & Cellular Proteomics : MCP·Sergey I KovalchukAdelina Rogowska-Wrzesinska
Jun 12, 2021·Analytical Chemistry·Xiaofei WangBo Zhang
Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
Jan 21, 2017·Analytical Chemistry·Stefan GaunitzMilos V Novotny
Jan 21, 2017·Analytical Chemistry·Xiaofeng ZhaoMilton L Lee
Oct 23, 2019·Analytical Chemistry·Alexander S KaplitzJames P Grinias

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