Capillary liquid chromatography separations using non-porous pillar array columns

Journal of Chromatography. a
Wim De MalscheFrederic Lynen

Abstract

We report on a series of explorative experiments wherein a non-porous pillar array column (NP-PAC) is coupled to a commercial capillary LC instrument. The performance of the system was evaluated by both non-retained and retained experiments using several types of samples. In order to minimize interfacing related dispersion, relatively large pillars (d(p)=11 μm) were defined so that a considerable depth could be achieved (50 μm), resulting in an equivalent cylindrical internal diameter of 252 μm. Connecting 20 channel tracks of 1mm wide and 7 cm long by previously developed distributor-based turns, a large channel length (1.4m) and volume (28μl) could be achieved without compromising the separation performance excessively. Establishing a van Deemter curve under non-retained conditions with off-chip injection and detection, a minimal plate height of 13 μm was established, resulting in some 100,000 plates obtained in 30 min. To demonstrate the practical applicability of the NP-PAC, high pressure operation was applied to perform a number of example separations (parabens, phenones, sulfonamides, steroids and BSA digest) during a continuous operation period of 3 months wherein some 500 injections were performed. In the gradient mode,...Continue Reading

References

Jul 26, 2005·Journal of Chromatography. a·Uwe D Neue
Jun 19, 2008·Analytical Chemistry·Wim De MalscheGert Desmet
Jun 22, 2010·Journal of Chromatography. a·Joris Vangelooven, Gert Desmet

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Citations

Nov 20, 2012·Analytical Chemistry·Gert Desmet, Sebastiaan Eeltink
Oct 23, 2012·Journal of Chromatography. a·Simon ForsterStephan Altmaier
Jul 4, 2012·Journal of Separation Science·Wim De MalscheGert Desmet
Dec 17, 2014·Journal of Chromatography. a·Konstantin N Smirnov, Oleg A Shpigun
Dec 21, 2013·The Analyst·David A CollinsBrett Paull

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