Capillary microsampling of whole blood for mouse PK studies: an easy route to serial blood sampling

Bioanalysis
Walter KorfmacherThomas O'Shea

Abstract

Capillary microsampling (CMS) of 8 µl of blood provides a methodology that can be utilized for serial sampling in drug discovery mouse PK studies. Blood CMS sample results were compared to plasma sample results for three compounds (with expected Cb/Cp of 1 to 2) and found to be similar. In addition, for three compounds, blood CMS results were found to be equivalent to results generated with standard whole blood sampling. In a 5-day repeated dose PK study, four mice were dosed (IV) daily and sampled on both day one and day five using blood CMS procedure. Blood CMS using 8 µl glass capillary microsamples provides a straightforward and effective approach for mouse serial blood sampling.

References

Oct 29, 2005·Drug Discovery Today·Walter A Korfmacher
Sep 23, 2008·Journal of Pharmaceutical Sciences·Sean X PengWilliam Hageman
Jun 26, 2013·Bioanalysis·Michael J SpreadboroughAmanda Wilson
Jan 23, 2014·Drug Discovery Today·Kathryn ChapmanAmanda Wilson
Jan 28, 2014·Pharmaceutical Research·Alison P JoyceDenise M O'Hara

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Citations

Jul 15, 2015·Journal of Pharmacological and Toxicological Methods·Walter KorfmacherThomas O'Shea
May 24, 2021·Pediatric Research·Yarmarly Guerra ValeroSuzanne L Parker

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