Abstract
Capillary plexuses are commonly regarded as reliable networks for blood flow and robust oxygen delivery to hypoxia sensitive tissues. They have high levels of redundancy to assure adequate blood supply when one or more of the capillaries in the network are blocked by a clot. However, despite having extensive capillary plexuses, many vital organs are often subject to secondary organ injury in patients with severe inflammation. Recent studies have suggested that neutrophils play a role in this pathology, even though their precise contribution remains elusive. Here we investigate the effect of chromatin fibres released from overly-activated neutrophils (neutrophil extracellular traps, NETs) on the flow of blood through microfluidic networks of channels replicating geometrical features of capillary plexuses. In an in vitro setting, we show that NETs can decouple the traffic of red blood cells from that of plasma in microfluidic networks. The effect is astonishingly disproportionate, with NETs from less than 200 neutrophils resulting in more than half of a 0.6 mm(2) microfluidic network to become void of red blood cell traffic. Importantly, the NETs are able to perturb the blood flow in capillary networks despite the presence of ant...Continue Reading
References
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Jul 11, 2006·Lancet·K A BrownD F Treacher
Jan 9, 2009·Kidney International·Alaa S AwadMark D Okusa
Aug 3, 2010·Nature Medicine·Steffen MassbergBernd Engelmann
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobias A FuchsDenisa D Wagner
Jun 28, 2011·The American Journal of Pathology·Teluguakula NarasarajuVincent T Chow
Nov 3, 2011·Journal of Thrombosis and Haemostasis : JTH·A BrillDenisa D Wagner
Mar 28, 2012·The Journal of Experimental Medicine·Marie-Luise von BrühlSteffen Massberg
Oct 30, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Maurits L van MontfoortSacha Zeerleder
Dec 12, 2012·American Journal of Respiratory and Critical Care Medicine·Simon T AbramsCheng-Hock Toh
Mar 22, 2014·Journal of the American Society of Nephrology : JASN·Rafael KramannBenjamin D Humphreys
Sep 14, 2014·Seminars in Nephrology·Rafael Kramann, Benjamin D Humphreys
Feb 11, 2015·European Heart Journal·Dimitrios A StakosKonstantinos Ritis
Mar 18, 2015·Lancet·Jessica L Mega, Tabassome Simon
May 2, 2015·The Lancet Infectious Diseases·Jonathan CohenEric Pelfrene
Jun 16, 2015·Nature Medicine·Siu Ling WongDenisa D Wagner
Oct 29, 2015·Critical Care Medicine·Yoshitaka InoueDaniel Irimia
Citations
Apr 10, 2018·American Journal of Respiratory and Critical Care Medicine·Katherine CrawfordHollis R O'Neal
May 20, 2016·Journal of Leukocyte Biology·Daniel Irimia, Felix Ellett
Aug 25, 2019·Heart and Vessels·Riha ShimizuTakanori Yasu
Jun 2, 2017·Scientific Reports·Galateja JordakievaErika Jensen-Jarolim
May 10, 2017·PloS One·Tamara HoppenbrouwersMoniek P M de Maat
Sep 11, 2019·The British Journal of Surgery·R J DinsdaleP Harrison
Nov 21, 2017·Journal of Thrombosis and Haemostasis : JTH·X YuS L Diamond
Nov 20, 2018·Scientific Reports·Masayuki OtawaraDaniel Irimia
Jun 22, 2019·Advanced Biosystems·Maedeh RoushanDaniel Irimia
Aug 21, 2020·Science·François BinetPrzemyslaw Sapieha
Apr 10, 2021·Burns and Trauma·Jon HazeldinePaul Harrison