Capillary Vibrating Sharp-Edge Spray Ionization (cVSSI) for Voltage-Free Liquid Chromatography-Mass Spectrometry

Journal of the American Society for Mass Spectrometry
Nandhini RanganathanPeng Li

Abstract

Here, we report a continuous flow-based ionization method, capillary vibrating sharp-edge spray ionization (cVSSI), that nebulizes liquid sample directly at the outlet of a capillary without using high-speed nebulization gas or a high electrical field. cVSSI is built upon the recently reported VSSI principle which nebulizes bulk liquid using vibrating sharp-edges. By attaching a short piece of fused silica capillary on top of the vibrating glass slide in VSSI, liquid is nebulized at the outlet of the capillary as the result of the vibration. Utilizing standard 360-μm OD/100-μm ID capillary, cVSSI works with a wide range of flow rates from 1 μL/min to 1 mL/min. The power consumption is as low as 130 mW. ESI-like MS spectra are obtained for small molecules, peptides, and proteins. Five orders of magnitude linear response for acetaminophen solution is achieved with a limit of detection (LOD) of 3 nM. cVSSI is also demonstrated to be compatible with LC-MS analysis. Two LC-MS applications are demonstrated with cVSSI: (1) separation and detection of a mixture of small molecules and (2) bottom-up proteomics using a protein digest. A mixture of nine common metabolites was appropriately separated and detected using LC-cVSSI-MS. In the b...Continue Reading

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Citations

Aug 20, 2019·Journal of Mass Spectrometry : JMS·Ya-Nan YaoBin Hu
Jul 22, 2020·Rapid Communications in Mass Spectrometry : RCM·Nandhini RanganathanPeng Li
Aug 29, 2020·Journal of the American Society for Mass Spectrometry·Kinkini Udara JayasundaraStephen J Valentine
Dec 4, 2020·Journal of the American Society for Mass Spectrometry·Khoa HoangMilan Pophristic

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