Capillary zone electrophoresis of glycopeptides under controlled electroosmotic flow conditions coupled to electrospray and matrix-assisted laser desorption/ionization mass spectrometry

Electrophoresis
Sabine AmonAndreas Rizzi

Abstract

Defined conditions of EOF along with different pH values of the BGE were compared for the purpose of analyzing glycopetides by CZE coupled to MS (CZE-MS). Hyphenation to MS involved ESI as well as MALDI, and single-stage and multistage MS were applied. Variation of the EOF was accomplished by selecting appropriate coatings for the capillary, namely hexadimethrine bromide (HDMB) and HDMB/dextran sulfate. A high and reproducible anodic and cathodic EOF, respectively, was obtained in both approaches, allowing the detection of analytes with net positive as well as negative charge in one single run. Thus, a fast and sensitive determination of the glycopeptides in a tryptic digest of antithrombin, chosen as a test sample, was achieved. Ionization suppression effects, a phenomenon typically observed with glycopeptides in MS analysis, were minimized thanks to separation from other peptides present. The high stability of the coatings permitted the generation of mass spectra without interfering peaks originating from the coating polymers. The high EOF generated by the coatings facilitated the maintenance of a stable spray when coupling to ESI-MS, and a stable CZE current when working with a sheath flow-assisted analyte deposition onto MA...Continue Reading

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Citations

May 20, 2008·Analytical Chemistry·Vratislav KostalEdgar A Arriaga
Mar 23, 2010·Journal of Chromatography. a·Ondrej Pes, Jan Preisler
Feb 14, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bernhard AntesAndreas Nechansky
Oct 20, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Manfred WuhrerCornelis H Hokke
Nov 14, 2006·Journal of Chromatography. a·Javier Hernández-BorgesAlejandro Cifuentes
May 15, 2009·Electrophoresis·Aline StaubJean-Luc Veuthey
Apr 15, 2008·Electrophoresis·Miguel HerreroAlejandro Cifuentes
Nov 30, 2007·Electrophoresis·Václav Kasicka
Apr 11, 2009·Journal of Separation Science·Bryan R Fonslow, John R Yates
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin
Jul 15, 2011·Rapid Communications in Mass Spectrometry : RCM·Estela GiménezVictoria Sanz-Nebot
Feb 1, 2011·Proteomics. Clinical Applications·Harald Mischak, Joost P Schanstra
Jul 30, 2014·Electrophoresis·Michael BiacchiEmmanuelle Leize-Wagner
Feb 18, 2010·Electrophoresis·Stefan BachmannGünther K Bonn
Dec 30, 2009·Electrophoresis·Eulsik OhYoung Sook Yoo
Dec 12, 2017·Mass Spectrometry Reviews·Qiwei ZhangJianjun Li
Mar 20, 2018·Chemical Reviews·L Renee RuhaakCarlito B Lebrilla

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