Capillary zone electrophoresis with fluid-impervious polymer tubing inside a fused-silica capillary

Journal of Chromatography. a
X Huang, C Horváth

Abstract

A fused-silica capillary was used as a mold in the in situ formation of, and as a sheath for, a highly crosslinked poly(styrene-divinylbenzene) inner tubing having 3-5 microns wall thickness. This 'tube-in-the-tube' construction with the thin-walled, fluid-impervious polymer inner tubing precluded contact between the aqueous buffer solution and the inner wall of the fused-silica capillary. As a result, this structure withstood long-term treatment with highly alkaline solutions without deterioration. In order to hydrophilize the inner surface of the polymer tube, a polyoxyethylene oligomer was grafted to its inner wall and subsequently crosslinked. The inner tube with such hydrophilic coating was also stable to hydrolytic attack by 1 M NaOH. Although the nonpolar polymeric inner surface generated electrosmotic flow as if it had some fixed negative charges, the flow velocity became almost negligibly small once it had been hydrophilized. As illustrated by electropherograms, the hydrophilization of the polymeric inner tube greatly facilitated the CZE of basic proteins in the pH range from 3 to 6 without the need for additives in the electrophoretic medium to mask the silanol groups at the surface of quartz capillaries.

References

Jun 19, 1985·Journal of Chromatography·Z el Rassi, C Horváth

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Citations

Sep 10, 2004·Journal of Separation Science·Michał Szumski, Bogusław Buszewski
Feb 23, 2007·Journal of Separation Science·Michal Szumski, Boguslaw Buszewski
Oct 27, 2007·Journal of Separation Science·Zdenka KucerováPavel Jandera
Apr 12, 2001·Electrophoresis·P G RighettiL Castelletti
Apr 12, 2001·Electrophoresis·J Horvath, V Dolník
Feb 16, 2007·Electrophoresis·Fernando BenaventeThomas Hankemeier
May 9, 2012·Journal of Chromatography. a·Mei HeD Jed Harrison
Sep 19, 2002·Journal of Chromatography. a·Shuhong ZhangCsaba Horváth
Jun 1, 2002·Analytical Chemistry·Constantina P KapnissiIsiah M Warner
Jun 29, 1999·Analytical Chemistry·W M DraperP R Michael
Jul 1, 2005·Analytical Chemistry·Tamás VigassyErnö Pretsch

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