PMID: 16524108Mar 10, 2006Paper

Capillary zone electrophoretic determination of phenolic compounds in chess (Bromus inermis L.) plant extracts

Journal of Separation Science
D SterbováVlastimil Kubán

Abstract

A simple CZE method for quantification of phenolic compounds (vanillin, cinnamic, sinapic, chlorogenic, syringic, ferulic, benzoic, p-coumaric, vanillic, p-hydroxybenzoic, rosmarinic, caffeic, gallic and protocatechuic acids) in less than 10 min using 20 mM sodium tetraborate (pH 9.2) with 5% v/v methanol as a BGE and with UV detection at 254 nm is described. The LODs (3 S/N) ranged between 0.02 and 0.12 microg/ mL. Repeatabilities (RSDs) were 0.66-1.8 and 1.56-4.23% for migration times and peak areas (n = 5), respectively. The method was applied to the determination of phenolic compounds in chess (Bromus inermis L.) after Soxhlet extraction and purification of the crude extracts with SPE procedures. The results compared well with those obtained by liquid chromatographic method. B. inermis was found as a suitable model plant containing a broad spectrum of phenolic compounds in easily detectable concentrations and as a potential source of antioxidants.

References

Dec 22, 1999·Electrophoresis·H J Issaq
Mar 29, 2000·Journal of Chromatography. a· Rodríguez-DelgadoF J García Montelongo
Jul 14, 2001·Journal of Pharmaceutical and Biomedical Analysis·G Zgórka, K Głowniak
Nov 21, 2001·Journal of Agricultural and Food Chemistry·W Zheng, S Y Wang
Jan 30, 2002·Journal of Pharmaceutical and Biomedical Analysis·Leena Suntornsuk
Nov 24, 2004·Journal of Chromatography. a·Marian Naczk, Fereidoon Shahidi

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