Capn5 Expression in the Healthy and Regenerating Zebrafish Retina

Investigative Ophthalmology & Visual Science
Cagney E Coomer, Ann C Morris

Abstract

Autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) is a devastating inherited autoimmune disease of the eye that displays features commonly seen in other eye diseases, such as retinitis pigmentosa and diabetic retinopathy. ADNIV is caused by a gain-of-function mutation in Calpain-5 (CAPN5), a calcium-dependent cysteine protease. Very little is known about the normal function of CAPN5 in the adult retina, and there are conflicting results regarding its role during mammalian embryonic development. The zebrafish (Danio rerio) is an excellent animal model for studying vertebrate development and tissue regeneration, and represents a novel model to explore the function of Capn5 in the eye. We characterized the expression of Capn5 in the developing zebrafish central nervous system (CNS) and retina, in the adult zebrafish retina, and in response to photoreceptor degeneration and regeneration using whole-mount in situ hybridization, FISH, and immunohistochemistry. In zebrafish, capn5 is strongly expressed in the developing embryonic brain, early optic vesicles, and in newly differentiated retinal photoreceptors. We found that expression of capn5 colocalized with cone-specific markers in the adult zebrafish retina. We ...Continue Reading

References

Feb 7, 1997·Cell·M D JacobsonM C Raff
Apr 16, 1998·Nature Genetics·J H PostlethwaitW S Talbot
Nov 30, 1998·Science·A AmoresJ H Postlethwait
Mar 13, 1999·The Journal of Biological Chemistry·D E Wood, E W Newcomb
Aug 23, 2001·Trends in Molecular Medicine·Y Huang, K K Wang
Jun 14, 2002·Biochimica Et Biophysica Acta·Tao LuRonald L Mellgren
Apr 2, 2003·Vision Research·Deborah C Otteson, Peter F Hitchcock
Jun 12, 2003·Developmental Biology·James M Fadool
Jan 30, 2004·Molecular and Cellular Biology·Tanna FranzT Neil Dear
Oct 7, 2004·Biochemical and Biophysical Research Communications·Anuradhaa WaghrayKevin K W Wang
Jun 10, 2005·The New England Journal of Medicine·Mayana Zatz, Alessandra Starling
Nov 24, 2005·Investigative Ophthalmology & Visual Science·Ann C MorrisJames M Fadool
Jan 13, 2006·Journal of Neurochemistry·Francois Paquet-DurandPer Ekström
Jan 26, 2006·BMC Developmental Biology·Previn DuttPeter A Greer
Apr 25, 2006·The Journal of Biological Chemistry·Yinfei TanPeter A Greer
Dec 7, 2007·Molecular Reproduction and Development·Jeffrey M Gross, Brian D Perkins
Feb 6, 2008·Zebrafish·Peter F Hitchcock, Pamela A Raymond
Feb 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stephanie E Lepage, Ashley E E Bruce
Mar 20, 2008·Survey of Ophthalmology·M Azuma, T R Shearer
Nov 5, 2008·Molecular Neurobiology·Javier Sancho-PelluzFrançois Paquet-Durand
Jan 29, 2010·Investigative Ophthalmology & Visual Science·Wei HuangCynthia L Grosskreutz
Jun 15, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hiroyuki SorimachiYasuko Ono
May 15, 2012·Cardiovascular Research·Matthew A Smith, Rick G Schnellmann
Jun 12, 2013·Developmental Biology·Marie A Forbes-OsborneAnn C Morris
Jan 2, 2014·Human Molecular Genetics·Katherine J WertVinit B Mahajan
Jan 15, 2014·Progress in Retinal and Eye Research·Jenny R Lenkowski, Pamela A Raymond
Jun 5, 2014·Nature Reviews. Neuroscience·Daniel Goldman
Sep 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dina AggadJ Alex Parker
Dec 6, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lakshmi Pillai-KastooriAnn C Morris
Dec 1, 2015·Experimental Eye Research·Stephen G WilsonAnn C Morris
May 7, 2016·Investigative Ophthalmology & Visual Science·Kellie A SchaeferVinit B Mahajan
Jul 9, 2016·Clinical Ophthalmology·Abdourahman ChamVinit B Mahajan

❮ Previous
Next ❯

Citations

Jul 11, 2020·Scientific Reports·Cagney E CoomerAnn C Morris
Aug 30, 2020·Neural Regeneration Research·Matthew R KentJames G Patton
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Warlen Pereira PiedadeJakub K Famulski
Jan 24, 2021·Immunologic Research·Yueqi ChenFang Liu
Apr 4, 2021·Cells·Salvatore L StellaStephanie L Grillo
Apr 4, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Vimala BondadaJames W Geddes
Dec 2, 2021·Journal of Cell Science·Kayla F Titialii-Torres, Ann C Morris

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
in vitro transcription
dissecting
electrophoresis
confocal microscopy
Confocal

Software Mentioned

BLAST

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.