Abstract
Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers. Here, we are the first to evaluate the expression of CapG in human CRCs. To investigate the expression levels of CapG in human tissue array by immunohistochemistry (IHC) staining. Moreover, the mRNA and protein levels were also confirmed in four CRC cell lines and determined using real-time RT-PCR and Western blotting. Finally, a Matrigel transwell invasion assay was used to evaluate the invasion ability in CapG high or low expression cells. We demonstrated that CapG could be determined in the normal colon tissue and human CRC specimens. However, CapG was significantly overexpressed in the mCRC specimens compared with that in CRC specimens and normal cases. It was also detectable in the four CRC cell lines ...Continue Reading
References
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·P SilacciD Hayoz
Jun 13, 2006·Oncogene·A WatariM Yutsudo
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Malte Renz, Jörg Langowski
Feb 18, 2011·Nature Reviews. Cancer·Alexander NürnbergRobert Grosse
Sep 13, 2011·The Tohoku Journal of Experimental Medicine·Fangchun ShaoKejing Ying
Dec 23, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Wang-Yu ZhuYong-Kui Zhang
Aug 29, 2012·PloS One·Jingli ZhuoCelestial T Yap
Jun 21, 2013·Journal of Proteome Research·Hiroshi IchikawaTadashi Kondo
May 8, 2014·BioMed Research International·J GlaserM C Fleisch
Jan 13, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kohei HashimotoNaoya Masumori
Dec 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wenli Xing, Chun Zeng
Jan 14, 2016·Journal of the National Cancer Institute·Jules A WestbrookJanet E Brown
Jan 21, 2017·International Journal of Molecular Sciences·Inés MármolMaría Jesús Rodriguez Yoldi
Feb 17, 2017·Therapeutic Advances in Medical Oncology·Rachel Riechelmann, Axel Grothey