Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure

PLoS Neglected Tropical Diseases
Jinhee YiPaul Keim

Abstract

Burkholderia pseudomallei causes melioidosis, a common source of pneumonia and sepsis in Southeast Asia and Northern Australia that results in high mortality rates. A caprine melioidosis model of aerosol infection that leads to a systemic infection has the potential to characterize the humoral immune response. This could help identify immunogenic proteins for new diagnostics and vaccine candidates. Outbred goats may more accurately mimic human infection, in contrast to the inbred mouse models used to date. B. pseudomallei infection was delivered as an intratracheal aerosol. Antigenic protein profiling was generated from the infecting strain MSHR511. Humoral immune responses were analyzed by ELISA and western blot, and the antigenic proteins were identified by mass spectrometry. Throughout the course of the infection the assay results demonstrated a much greater humoral response with IgG antibodies, in both breadth and quantity, compared to IgM antibodies. Pre-infection sera showed multiple immunogenic proteins already reactive for IgG (7-20) and IgM (0-12) in most of the goats despite no previous exposure to B. pseudomallei. After infection, the number of IgG reactive proteins showed a marked increase as the disease progressed....Continue Reading

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
ELISA
electrophoresis
PCR
ELISAs

Software Mentioned

BLAST
4000 series Explorer
Protein Pilot
ExPASy
Compute pI / Mw
Prism
GraphPad
Melanie

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