Capsaicin causes robust reduction in glycinergic transmission to rat hypoglossal motor neurons via a TRPV1-independent mechanism

Journal of Neurophysiology
Prajwal P Thakre, Mark C Bellingham

Abstract

The effect of capsaicin on glycinergic synaptic transmission to juvenile rat hypoglossal motor neurons in acute brainstem slices was evaluated in the presence of TTX. Capsaicin caused a robust decrease in miniature IPSC frequency, amplitude, and half-width, showing that this effect is independent of action potential generation. In the presence of capsazepine, a classic TRPV1 antagonist, capsaicin was still able to reduce spontaneous inhibitory postsynaptic current (IPSC) amplitude and frequency. We further investigated whether the effect of capsaicin on glycinergic transmission to hypoglossal motor neurons is pre- or postsynaptic in nature by recording pairs of evoked IPSCs. Interestingly, capsaicin also reduced evoked IPSC amplitude without affecting paired-pulse ratio, indicating a postsynaptic mechanism of action. Significant reduction was also observed in evoked IPSC half-width, rise time, and decay tau. We also show that capsaicin does not have any effect on either transient (It) or sustained (Is) potassium currents. Finally, we also show that the hyperpolarization-activated cationic current (Ih) also remains unchanged after capsaicin application. NEW & NOTEWORTHY Capsaicin reduces the amplitude of quantal and evoked glyci...Continue Reading

References

Jun 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J E RemmersA M Anch
Jan 1, 1980·Progress in Neurobiology·A A Lowe
Jan 1, 1995·Advances in Experimental Medicine and Biology·A J BergerF Viana
Apr 4, 2000·Physiological Reviews·J C ReklingJ L Feldman
Apr 3, 2001·Annual Review of Neuroscience·M J Caterina, D Julius
Sep 3, 2002·The Journal of Physiology·Silvia MarinelliMark Connor
Jan 25, 2003·Brain Research·Kyungpyo ParkJoong-Soo Kim
Mar 11, 2003·Journal of Neurophysiology·L Liu, S A Simon
Jun 22, 2005·Molecular Pharmacology·J A LundbaekO S Andersen
May 5, 2006·Neuroscience Letters·Attila KöfalviRodrigo A Cunha
Jul 17, 2007·Brain Research·Xuehong CaoLieju Liu
Jun 4, 2010·The Journal of Physiology·Jamie JohnstonConny Kopp-Scheinpflug
Feb 22, 2011·Neurochemistry International·Tae-Hee LeeSang-Yoon Nam
Jun 19, 2013·Respiratory Physiology & Neurobiology·Jan-Marino RamirezNanduri R Prabhakar
Mar 5, 2014·Cellular and Molecular Neurobiology·Rong YangLieju Liu
Jan 8, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthew J FogartyPeter G Noakes
Oct 7, 2016·American Journal of Respiratory Cell and Molecular Biology·Jun RenJohn J Greer

❮ Previous
Next ❯

Citations

Feb 11, 2020·The Journal of Physiological Sciences : JPS·Koji ObataMiyako Takaki
Sep 29, 2020·Frontiers in Pharmacology·Eslam El NebrisiMurat Oz
Aug 9, 2020·European Journal of Pharmacology·Leonardo Gomes Braga FerreiraRobson Xavier Faria

❮ Previous
Next ❯

Software Mentioned

Clampfit
GraphPad Prism
Excel
Solver

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Regional Anesthesia and Pain Medicine
R E Wachtel
Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Pompy PatowaryPronobesh Chattopadhyay
Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
J Hammer, M Führer
© 2022 Meta ULC. All rights reserved