PMID: 9178350Mar 26, 1997Paper

Capsaicin-evoked release of pituitary adenylate cyclase activating peptide (PACAP) and calcitonin gene-related peptide (CGRP) from rat spinal cord in vivo

Regulatory Peptides
Y Z ZhangR Håkanson

Abstract

Capsaicin-evoked release of pituitary adenylate cyclase activating peptide (PACAP)-like immunoreactivity (LI) from rat spinal cord was examined in vivo. In anaesthetized rats, a catheter was inserted through the atlanto-occipital membrane into the subarachnoid space at the level of the sacral spinal cord for infusion of artificial cerebrospinal fluid. Another catheter was placed in the cisternal opening for outflow. Blood pressure was monitored and kept stable during the experiment. Perfusion samples were analyzed for PACAP and calcitonin gene-related peptide (CGRP) by radioimmunoassay. The addition of capsaicin (10 microM) to the perfusate elevated the concentrations of PACAP-27-LI in the artificial cerebrospinal fluid by 177%, PACAP-38-LI by 93% and CGRP-LI by 692%. In view of the presence of PACAP-immunoreactive nerve fibres in the superficial layers of the dorsal horn and the expression of PACAP in the small sized neurons in the dorsal root ganglia, the findings suggest that PACAP is released into the artificial cerebrospinal fluid from C-fibres in the spinal cord. PACAP conceivably plays a modulating role in nociception.

References

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Citations

May 1, 2010·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Henrik W SchytzMessoud Ashina
Feb 13, 1999·Progress in Neurobiology·M J Millan
Apr 20, 2006·Critical Reviews in Food Science and Nutrition·M N Satyanarayana
Dec 5, 2008·Brain : a Journal of Neurology·Henrik Winther SchytzMessoud Ashina
Dec 22, 2009·The Journal of Urology·Victor May, Margaret A Vizzard
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Sep 25, 1999·Japanese Journal of Pharmacology·T Sasamura, Y Kuraishi
Feb 18, 2018·The Journal of Headache and Pain·Håkan AshinaMessoud Ashina
Mar 11, 2018·The Journal of Headache and Pain·Lars EdvinssonLászló Vécsei
Jan 25, 2018·The Journal of Pharmacology and Experimental Therapeutics·Ichiro TakasakiTakashi Kurihara
Jul 10, 2019·Current Topics in Medicinal Chemistry·Chenyi LiaoJianing Li

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