Abstract
Capsaicin, the pungent agent in chili peppers, has been shown to act as a tumor-suppressor in cancer. In our previous study, capsaicin was shown to induce apoptosis in the rat pheochromocytoma cell line (PC12 cells). Thus, the aim of the present study was to determine the potential mechanism by which capsaicin induces apoptosis. We treated PC12 cells with 50, 100 and 500 µM capsaicin and measured the reticular calcium content and expression of the reticular calcium transport systems. These results were correlated with endoplasmic reticulum (ER) stress markers CHOP, ATF4 and X-box binding protein 1 (XBP1), as well as with apoptosis induction. We observed that capsaicin decreased reticular calcium in a concentration-dependent manner. Simultaneously, expression levels of the sarco/endoplasmic reticulum pump and ryanodin receptor of type 2 were modified. These changes were accompanied by increased ER stress, as documented by increased stress markers. Thus, from these results we propose that in PC12 cells capsaicin induces apoptosis through increased ER stress.
References
Jan 1, 1990·Chemico-biological Interactions·A KalénG Dallner
Feb 17, 2001·Circulation Research·B LevkauG Breithardt
Feb 12, 2004·Cancer Research·Keisuke ItoMasahiro Kizaki
Aug 24, 2004·The Journal of Biological Chemistry·Hirohito Yamaguchi, Hong-Gang Wang
Dec 2, 2004·Nature Reviews. Cancer·Yanjun Ma, Linda M Hendershot
Mar 31, 2007·Circulation Research·Héctor H Valdivia
Sep 15, 2007·Antioxidants & Redox Signaling·Claudio A Hetz
Mar 29, 2008·Cell Death and Differentiation·V LabiA Villunger
Jun 6, 2008·Biochemical and Biophysical Research Communications·Ana María SánchezInés Díaz-Laviada
Nov 13, 2008·International Journal of Cancer. Journal International Du Cancer·Simone Fulda
Jul 18, 2009·Drug News & Perspectives·Ayesha N ShajahanRobert Clarke
Jul 14, 2010·FEBS Letters·O Teijido, L Dejean
Jul 14, 2010·PLoS Biology·Sanjeev GuptaAfshin Samali
Feb 12, 2011·Carcinogenesis·Sara CaprodossiGiorgio Santoni
Feb 26, 2011·Current Pharmaceutical Design·Guyla G JohnsonMaurizio Grimaldi
Jul 5, 2011·Experimental Hematology·Feng-Ming WangHongjiao Ouyang
Aug 3, 2011·Biotechnology and Bioengineering·Anirikh ChakrabartiJeffrey D Varner
Sep 1, 2011·American Journal of Physiology. Endocrinology and Metabolism·Yuria SaitoKazuhiro Takekoshi
Nov 9, 2011·Biochimica Et Biophysica Acta·Komal JhaveriGabriela Chiosis
Mar 13, 2012·International Journal of Cancer. Journal International Du Cancer·Karel PacakOlga Krizanova
Dec 28, 2012·Journal of Pharmacological Sciences·Tatsuhiko IkedaKazuhiro Takekoshi
Jan 25, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chien-Chih OuJah-Yao Liu
Mar 5, 2013·PloS One·Alessio GiubellinoKarel Pacak
Mar 29, 2013·PloS One·Sabine LotteauFabien Van Coppenolle
Apr 16, 2013·Acta Physiologica·L LencesovaO Krizanova
Apr 16, 2013·Channels·Naoki ItoShin'ichi Takeda
May 22, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yin-Quan TangShamala Devi Sekaran
Citations
Jan 18, 2018·Scientific Reports·Ryosuke KidaMasayuki Funaba
Feb 15, 2019·FEBS Open Bio·Rongqi HuangZhiyuan Li
Jun 18, 2020·International Journal of Molecular Sciences·Kevin ZhaiDietrich Büsselberg
May 3, 2016·Radiation Research·Feifei LiQiang Li
Dec 23, 2016·Physiological Research·D LekesP Babula