Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression

Oncotarget
Marisa GranatoMara Cirone

Abstract

Capsaicin, the pungent alkaloid of red pepper has been extensively studied for its many properties, especially the anti-inflammatory and anti-oxidant ones. It binds to vanilloid receptor 1, although it has been reported to be able to mediate some effects independently of its receptor. Another important property of Capsaicin is the anticancer activity against highly malignant tumors, alone or in combination with other chemotherapeutic agents. In this study, we found that Capsaicin induced an apoptotic cell death in PEL cells correlated with the inhibition of STAT3. STAT3 pathway, constitutively activated in PEL cells, is essential for their survival. By STAT3 de-phosphorylation, Capsaicin reduced the Mcl-1 expression level and this could represent one of the underlying mechanisms leading to the Capsaicin-mediated cell death and autophagy induction. Next, by pharmacological or genetic inhibition, we found that autophagy played a pro-survival role, suggesting that its inhibition could be exploited to increase the Capsaicin cytotoxic effect against PEL cells. Finally, we show that Capsaicin induced DAMP exposure, as for an immunogenic cell death, directly promoted DC activation and, more importantly, that it counteracted the immune...Continue Reading

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Citations

Nov 29, 2016·Oncotarget·Nicola Di DanieleAntonino De Lorenzo
Sep 8, 2016·Journal of Experimental & Clinical Cancer Research : CR·Alessia GarufiGabriella D'Orazi
Apr 24, 2020·Computational and Structural Biotechnology Journal·Pingli WeiLingjun Li
Jan 13, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Yuewen SunXiaoyong Wang
Feb 27, 2021·Molecular Cancer Therapeutics·Maria Saveria Gilardini MontaniMara Cirone

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Methods Mentioned

BETA
FACS
Flow cytometry
electrophoresis
transfection
chemical treatment
light microscopy

Software Mentioned

Image J

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