Capsid size determination in the P2-P4 bacteriophage system: suppression of sir mutations in P2's capsid gene N by supersid mutations in P4's external scaffold gene sid

Virology
K J KimE W Six

Abstract

The sid gene of the P2-dependent phage P4 provides an external scaffold so P2 N gene encoded protomers assemble as T = 4 capsids rather than as P2's T = 7 capsids. Mutations (sir) in the middle of N interfere with Sid's function. We describe a new P4 mutant class, nms ("supersid") mutations, which direct also P2 sir to provide small capsids. Three different nms mutations were located near the sid end, commingled with sid(-) mutations. Suppression of sir by nms is not allele-specific. Our results favor this interpretation of capsid size control: (i) sir mutations reduce pN protomer flexibility and thereby interfere with the generation of T = 4 compatible hexons; (ii) the C-termini of Sid molecules link up when forming the scaffold; nms mutations strengthen these Sid-Sid contacts and thus allow the scaffold to force even sir-type protomers to form T = 4 compatible hexons. Some related findings concern suppression of N ts mutations by P4.

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D ShoreR Goldstein
May 1, 1991·Virology·E W SixB H Lindqvist
May 24, 1991·Science·A LupasJ Stock
Apr 1, 1985·Journal of Virology·A RaimondiG Dehò
Jun 1, 1970·Journal of Virology·D H Walker, T F Anderson
Jul 1, 1969·The Journal of General Virology·G BertaniG Lindahl
Dec 1, 1969·Virology·G Lindahl
Sep 1, 1965·Journal of General Microbiology·I Sasaki, G Bertani
Jan 1, 1971·Molecular & General Genetics : MGG·B H Lindqvist
Nov 26, 1984·Nucleic Acids Research·C S Lin
Jan 1, 1982·Genetics·E C Cox, D L Horner
Oct 20, 1995·Virology·E Haggård-LjungquistE V Koonin
Jul 1, 1995·Journal of Bacteriology·B Julien, R Calendar
Aug 4, 1995·Journal of Molecular Biology·O J MarvikB H Lindqvist
Sep 1, 1993·Microbiological Reviews·B H LindqvistR Calendar
Mar 1, 1993·Protein Science : a Publication of the Protein Society·C M StultzT F Smith
Jan 1, 1996·Methods in Enzymology·A Lupas
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·T Dokland
Oct 1, 1958·Virology·G BERTANI, E SIX
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·D L CASPAR, A KLUG

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Citations

Apr 18, 2012·Journal of Structural Biology·Altaira D DearbornTerje Dokland
Nov 8, 2012·Virology·Gail E Christie, Terje Dokland
May 8, 2007·Virology·Gipsi Lima-MendezRaphaël Leplae
Oct 1, 2004·American Journal of Botany·François LutzoniRytas Vilgalys
Jun 8, 2019·Microbiology Spectrum·Richard P Novick

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