Capsulated cells of Aeromonas salmonicida grown in vitro have different functional properties than capsulated cells grown in vivo

Canadian Journal of Microbiology
R A Garduño, W W Kay

Abstract

When grown in vivo in the peritoneal cavity of rainbow trout, Aeromonas salmonicida produces a clearly defined capsule with virulence-related functions. Aeromonas salmonicida grown in vitro in a glucose-rich medium (GRM) has also been reported to reproduce capsular material. Because in vitro mimicry of in vivo induced traits is highly desirable in vaccine design, the extent to which growth in GRM mimicked in vivo growth was examined. Antibodies specific to in vivo grown cells partially labeled the surface of GRM-grown cells, as well as two distinct proteins (81,700 and 41,000 Mr) in immunoblots of mutants with S-layer or lipopolysaccharide defects. GRM-grown strains showed an increased sensitivity to trout serum in contradistinction to the complete serum resistance of in vivo grown cells; as well, GRM-grown cells were more adherent to trout macrophages. Thus in spite of possessing some surface antigens normally expressed in vivo, cells grown on solid GRM did not possess all functional properties of in vivo grown cells.

References

Nov 1, 1992·Infection and Immunity·R A Garduño, W W Kay
Jan 1, 1991·Advances in Microbial Physiology·M Fletcher
Nov 1, 1989·Journal of Clinical Microbiology·V Lorian
Jun 1, 1982·Infection and Immunity·C B MunnT J Trust
Sep 1, 1993·Infection and Immunity·R A GarduñoW W Kay
Aug 1, 1993·Applied and Environmental Microbiology·R BonetF Congregado
Nov 1, 1993·Infection and Immunity·J C ThorntonW W Kay

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Citations

Feb 20, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M A PereiraA Almeida
Aug 10, 2000·Canadian Journal of Microbiology·R A GarduñoW W Kay
May 1, 1997·Microbial Pathogenesis·S MerinoF Congregado
Oct 8, 1997·Infection and Immunity·L B CorbeilT J Beveridge

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