Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms

Biotechnology and Bioengineering
Takahito NakagawaTadashi Matsunaga

Abstract

Bacterial magnetic particles (BMPs) were modified with 3-[2-(2-aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 x 10(4) surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 microg of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80 degrees C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated.

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, D Gillespie
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 25, 1994·Nucleic Acids Research·T L HawkinsC Santillan
Oct 15, 1996·Analytical Chemistry·T MatsunagaN Nakamura
Feb 28, 2002·Journal of Biotechnology·Brandon YozaTadashi Matsunaga
Mar 14, 2002·Electrophoresis·Kelley A WolfeJames P Landers
Mar 5, 2003·Journal of Biotechnology·Brandon YozaTadashi Matsunaga
Jan 25, 2005·Journal of Biotechnology·Takahito NakagawaTadashi Matsunaga
Oct 20, 2005·Journal of Bioscience and Bioengineering·Brandon YozaTadashi Matsunaga

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Citations

Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Ceren TürkcanAdil Denizli
Mar 22, 2008·Analytical Chemistry·Małgorzata A WitekSteven A Soper
Apr 15, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Laurent BouffierMatthew J Rosseinsky
Jun 19, 2008·Journal of the Royal Society, Interface·Atsushi ArakakiTadashi Matsunaga
Oct 28, 2008·Applied and Environmental Microbiology·Tsuyoshi TanakaTadashi Matsunaga
Jun 24, 2008·Applied and Environmental Microbiology·Yoshiaki MaedaTadashi Matsunaga
Dec 26, 2015·Colloids and Surfaces. B, Biointerfaces·Yoshiaki MaedaTsuyoshi Tanaka
Apr 4, 2009·Journal of Integrative Plant Biology·Ji-Feng ChenShao-Hua Li
Jul 24, 2012·Drug Discovery Today·Md Mahbubor Rahman, Abdelhamid Elaissari
Nov 30, 2011·Electrophoresis·Rawi RamautarGovert W Somsen
Jul 9, 2009·Journal of Separation Science·Lijia LiuYibing Zhang
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jungkyu KimBruce K Gale
Nov 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Ligang GaiXincheng Chen
May 31, 2013·Journal of Separation Science·Bohuslav Rittich, Alena Spanová
May 17, 2017·Chemistry, an Asian Journal·Xuan-Hung PhamBong-Hyun Jun
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Tsuyoshi TanakaTadashi Matsunaga
Jun 15, 2021·ACS Applied Materials & Interfaces·Daryl W YeeJulia R Greer

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