Capture and release of genomic DNA by PEI modified Fe3 O4 /Au nanoparticles

Materials Science & Engineering. C, Materials for Biological Applications
Han-Wen SunDunqing Wang

Abstract

Polyethylenimine (PEI) modified Fe3O4/Au nanoparticles were synthesized in aqueous solution and characterized by photo correlation spectroscopy (PCS) and vibrating sample magnetometer (VSM). The so-obtained Fe3O4/Au-PEI nanoparticles were capable of efficient electrostatic capture of DNA. The maximum amount of genomic DNA captured on 1.0mg Fe3O4/Au-PEI nanoparticles was 90μg. The DNA release behavior was studied and the DNA recovery from Fe3O4/Au-PEI nanoparticles approached 100% under optimal conditions. DNA extraction from mammalian cells using Fe3O4/Au-PEI nanoparticles was successfully performed. Up to approximately 43.1μg of high-purity (OD260/OD280 ratio=1.81) genomic DNA was extracted from 10mg of liver tissue. The results indicated that the prepared Fe3O4/Au-PEI nanoparticles could be successfully used for DNA capture and release.

Citations

Aug 24, 2018·Environmental Technology·Luiz Fernando Oliveira MaiaJairo Lisboa Rodrigues
Apr 19, 2019·Chemistry, an Asian Journal·Ming LuoJianguo Guan
May 31, 2013·Journal of Separation Science·Bohuslav Rittich, Alena Spanová
Apr 22, 2017·Journal of Applied Biomaterials & Functional Materials·Cunqing MaXin Wang
Jul 22, 2014·Chemistry : a European Journal·Henning LülfLuisa De Cola
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