PMID: 7542870Jul 15, 1995Paper

Capture by chemical crosslinkers provides evidence that integrin alpha IIb beta 3 forms a complex with protein tyrosine kinases in intact platelets

The Biochemical Journal
D J DorahyG F Burns

Abstract

Platelet activation is accompanied by a cascade of kinase reactions in which numerous specific proteins are phosphorylated on tyrosine. These events are strictly dependent upon functional activation of an integrin receptor, generally alpha IIb beta 3 (also known as glycoprotein IIb-IIIa). It is not known how alpha IIb beta 3 regulates protein tyrosine kinase activation and, in particular, neither this nor any other integrin has been shown to associate with a protein tyrosine kinase. We employed chemical crosslinking of intact platelets with the bifunctional reagents dithiobis(succinimidyl propionate) (DSP) (lipid-soluble) and dithiobis(sulphosuccinimidyl propionate) (DTSSP) (lipid-insoluble) followed by in vitro kinase assays of immunoprecipitated proteins to identify kinase activity associated with alpha IIb beta 3 in intact platelets. It was found that DSP but not DTSSP crosslinked kinase activity to alpha IIb beta 3, suggesting an internal association. In these immunoprecipitates from DSP-crosslinked platelets, the in vitro kinase reaction revealed a complex of several phosphoproteins in association with alpha IIb beta 3. This association was not seen when the resting platelets were lysed before crosslinking, indicating the ...Continue Reading

Citations

Jun 5, 1998·The Journal of Biological Chemistry·A L JenkinsD R Phillips
May 29, 2004·The Journal of Biological Chemistry·Mhairi J MaxwellShaun P Jackson
Jul 6, 2000·The Journal of Pathology·D E MacCallum, P A Hall
Feb 10, 2012·Journal of Veterinary Emergency and Critical Care·Robert Goggs, Alastair W Poole
Dec 1, 1995·Australian and New Zealand Journal of Medicine·M C BerndtR K Andrews
Jan 22, 2010·Journal of Cellular Biochemistry·Katarzyna A LechwardSusanne Falkner
Aug 19, 2018·Scientific Reports·Seock-Won YounHyo-Soo Kim
Jul 17, 1998·Neurobiology of Disease·G ThinakaranS S Sisodia
Jul 15, 1999·Biochemical and Biophysical Research Communications·M E BertagnolliG Y Stetsenko

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