Capture of endothelial cells under flow using immobilized vascular endothelial growth factor

Biomaterials
Randall J SmithStelios T Andreadis

Abstract

We demonstrate the ability of immobilized vascular endothelial growth factor (VEGF) to capture endothelial cells (EC) with high specificity under fluid flow. To this end, we engineered a surface consisting of heparin bound to poly-l-lysine to permit immobilization of VEGF through the C-terminal heparin-binding domain. The immobilized growth factor retained its biological activity as shown by proliferation of EC and prolonged activation of KDR signaling. Using a microfluidic device we assessed the ability to capture EC under a range of shear stresses from low (0.5 dyne/cm(2)) to physiological (15 dyne/cm(2)). Capture was significant for all shear stresses tested. Immobilized VEGF was highly selective for EC as evidenced by significant capture of human umbilical vein and ovine pulmonary artery EC but no capture of human dermal fibroblasts, human hair follicle derived mesenchymal stem cells, or mouse fibroblasts. Further, VEGF could capture EC from mixtures with non-EC under low and high shear conditions as well as from complex fluids like whole human blood under high shear. Our findings may have far reaching implications, as they suggest that VEGF could be used to promote endothelialization of vascular grafts or neovascularizatio...Continue Reading

References

Sep 30, 1992·Biochemical and Biophysical Research Communications·B I TermanP Böhlen
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Dec 29, 1989·Biochemical and Biophysical Research Communications·E TischerJ A Abraham
Dec 1, 1980·Analytical Biochemistry·P K SmithG T Hermanson
Oct 1, 1996·Cytokine & Growth Factor Reviews·M Klagsbrun, P A D'Amore
Apr 17, 1997·Nature·W Risau
Apr 30, 1998·Current Opinion in Cell Biology·E I Korpelainen, K Alitalo
Apr 16, 1999·Science·L E NiklasonR Langer
Dec 5, 2000·Current Opinion in Biotechnology·N Ferrara
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·X Li, U Eriksson
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·A H ZischJ A Hubbell
Mar 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea S Gobin, Jennifer L West
Nov 30, 2002·Trends in Pharmacological Sciences·Andreas Bikfalvi, Roy Bicknell
Apr 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Feilim Mac Gabhann, Aleksander S Popel
May 31, 2003·EMBO Reports·J Andrew McKeeChristopher M Counter
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Vikas PrabhakarLaura E Niklason
Dec 6, 2003·Biotechnology Progress·Andrea S Gobin, Jennifer L West
Jan 20, 2004·Journal of Molecular Biology·Hironobu YamashitaYasuo Kitagawa
Oct 1, 1993·Current Biology : CB·M Klagsbrun, S Soker
Dec 18, 2004·Biomaterials·Solitaire A DeLongJennifer L West
Sep 13, 2005·Lancet·Ruurd Torensma
Nov 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeremy N RichHenry S Friedman
Dec 7, 2005·Experimental Cell Research·Yin-Shan NgDavid T Shima
Feb 24, 2006·Nature Medicine·Nicolas L'HeureuxTodd N McAllister
May 17, 2006·Acta Biomaterialia·Danielle S W Benoit, Kristi S Anseth
Jun 20, 2006·Trends in Cardiovascular Medicine·Zhaodi Gong, Laura E Niklason
Jul 4, 2006·The Journal of Biological Chemistry·Sylvie Ricard-BlumFlorence Ruggiero
Mar 9, 2007·Blood Cells, Molecules & Diseases·Zaher K OtrockAli I Shamseddine
May 29, 2007·Microvascular Research·Arindel S R Maharaj, Patricia A D'Amore
Jun 1, 2007·The International Journal of Biochemistry & Cell Biology·Quoc T Ho, Calvin J Kuo
Jun 26, 2007·Nature Clinical Practice. Cardiovascular Medicine·Nicolas L'HeureuxTodd McAllister
Oct 5, 2007·The New England Journal of Medicine·Nicolas L'HeureuxLuis M de la Fuente
Dec 11, 2007·Journal of Biomedical Materials Research. Part a·Claude LeclercRichard Lo-Man

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Citations

Apr 14, 2016·Advanced Healthcare Materials·Jeremy J Glynn, Monica T Hinds
May 12, 2016·Advanced Materials·Małgorzata A WronskaJ Gerard Wall
Oct 4, 2016·Journal of Tissue Engineering and Regenerative Medicine·Emanuela S FiorettaSimon P Hoerstrup
Sep 29, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shurui LiaoXingyi Xie
Jan 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Randall J SmithStelios T Andreadis
Jul 5, 2017·Technology·Sindhu RowStelios T Andreadis
Mar 11, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ileana PirozziAnubhav Tripathi
Oct 14, 2019·Journal of Biomedical Materials Research. Part a·Xiuzhen WanJiang Yuan
Aug 15, 2020·Regenerative Biomaterials·Pan ZhaoJun Fan
Sep 11, 2020·Nature Reviews. Cardiology·Emanuela S FiorettaMaximilian Y Emmert
Mar 11, 2017·Journal of Cardiovascular Translational Research·Maximilian Y EmmertSimon P Hoerstrup
Apr 3, 2020·Nature Communications·Randall J SmithStelios T Andreadis
Aug 26, 2017·Advanced Healthcare Materials·Bastiaan D Ippel, Patricia Y W Dankers
Jul 22, 2018·International Journal of Molecular Sciences·Chih-Hsun LinJen-Her Lu
Feb 9, 2021·Advanced Functional Materials·Bita NasiriStelios T Andreadis
Jul 10, 2021·ACS Applied Materials & Interfaces·Andreia T PereiraInês C Gonçalves

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Methods Mentioned

BETA
coronary artery bypass
ELISA

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