Capture of p53 by electrodes modified with consensus DNA duplexes and amplified voltammetric detection using ferrocene-capped gold nanoparticle/streptavidin conjugates

Analytical Chemistry
Jianxiu WangFeimeng Zhou

Abstract

p53, a tumor suppressor protein and a transcription factor, is capable of inhibiting the growth of tumor cells by eliciting either cell-cycle arrest or apoptosis through a cascade of events. p53 binds sites within the promoters of several genes that conform to a sequence commonly defined as the consensus site. In more than 50% of cancer cases, the p53 gene has been found to be mutated and the p53 protein loses its ability to bind the consensus DNA. In this work, double-stranded (ds-) oligonucleotides (ODNs) containing the consensus site are immobilized onto gold electrodes to capture wild-type p53. The cysteine residues on the exterior of the p53 molecule were derivatized for the attachment of gold nanoparticle/streptavidin conjugates capped with multiple ferrocene (Fc) groups. Well-defined voltammetric peaks of high signal intensity were obtained, and p53 concentration as low as 2.2 pM was measured. The peak heights were found to be dependent on the surface density of the consensus ds-ODN, the sequence of the immobilized ODNs, and the p53 concentration. With base pair(s) in the full consensus binding sequence altered, the level of p53 binding was found to decrease sharply, and no p53 binding occurred at electrodes covered with...Continue Reading

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Citations

Oct 30, 2009·Analytical and Bioanalytical Chemistry·Ning XiaJianxiu Wang
May 28, 2013·Biosensors & Bioelectronics·Yingying DingMinghui Yang
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Sep 30, 2010·Journal of the American Chemical Society·Fan XiaAlan J Heeger
Nov 17, 2009·Advanced Drug Delivery Reviews·Sarit S AgastiVincent M Rotello
Feb 25, 2017·Journal of the American Chemical Society·Galina V DubachevaRalf P Richter
Sep 15, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Suprit DeolYoung-Seok Shon
May 27, 2010·The Analyst·Eli G Hvastkovs, Daniel A Buttry
Dec 6, 2013·Materials·Shigehiro Takahashi, Jun-Ichi Anzai
Jun 27, 2015·Chemical Reviews·Wen ZhouXiaoyuan Chen

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