Capture of single-stranded DNA assisted by oligonucleotide modules

Analytical Biochemistry
D O'mearaJ Lundeberg

Abstract

Real-time biospecific interaction analysis was employed to monitor direct capture of a hepatitis C virus (HCV) derived polymerase chain reaction (PCR) product by nucleic acid hybridization. Different formats for hybridization were used to study the interaction between a single-stranded HCV PCR product and capture oligonucleotides immobilized on a sensor chip via streptavidin-biotin chemistry. By employing a prehybridization step in solution with nonbiotin oligonucleotides complementary to the single-stranded target and adjacent to the immobilized probe, a significant capture was achieved in comparison to the low capture efficiency obtained using single immobilized probes (9-36 mer). High capture efficiencies were also observed when shorter immobilized probes were used in combination with strings of adjacently positioned prehybridized probes (i.e., modules). Interestingly, the introduction of single nucleotide gaps between prehybridized and/or immobilized probes dramatically reduced the capture efficiency. These results suggest that flexible systems for capture could be designed from libraries of short oligonucleotides (9 mers) used in module fashion, taking advantage of stacking interactions between the oligonucleotides. The po...Continue Reading

References

Dec 11, 1992·Science·J KieleczawaF W Studier
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·K R KhrapkoA D Mirzabekov
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J WahlbergM Uhlén
Oct 9, 1989·FEBS Letters·K R KhrapkoA D Mirzabekov
Aug 5, 1993·Nature·S P FodorC L Adams
Jan 1, 1995·Analytical Biochemistry·P NilssonP A Nygren
Jan 1, 1994·Journal of Medical Virology·L J van DoornW Quint
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L E KotlerL E Ulanovsky
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M BuckleH Buc
Apr 1, 1996·Journal of Clinical Microbiology·M StarkJ Lundeberg
Dec 31, 1995·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·M GotohM Tosu
Jan 1, 1997·Journal of Molecular Recognition : JMR·P NilssonP A Nygren
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown

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Citations

Aug 5, 2010·Journal of the American Chemical Society·Andrew E PrigodichChad A Mirkin
Apr 9, 2005·Nucleic Acids Research·Richard T Pon, Shuyuan Yu
Jul 26, 2002·Nucleic Acids Research·Deirdre O'MearaJoakim Lundeberg
Nov 30, 2010·Biochimica Et Biophysica Acta·Elena V BichenkovaKenneth T Douglas
Mar 27, 2007·The Journal of Molecular Diagnostics : JMD·Joseph C LiaoDavid A Haake
Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Jan 25, 2008·Applied Microbiology and Biotechnology·Daniel PiochThomas Schweder
Dec 6, 2008·Chemical Communications : Chem Comm·Bi-feng YuanZheng Tan
Nov 18, 2014·World Journal of Gastroenterology : WJG·Carolina V UlianaHideko Yamanaka
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hideyuki ArataMizuo Maeda
Jan 15, 2000·Journal of Biomolecular Structure & Dynamics·C M NiemeyerD Blohm
Aug 25, 2018·Journal of Pharmaceutical Analysis·Yinan WangJianzhong Lu
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Turková
May 29, 2000·Biosensors & Bioelectronics·B H SchneiderL V Howard
Aug 15, 1998·Journal of Clinical Microbiology·D O'MearaJ Lundeberg
Feb 22, 2002·The Journal of Organic Chemistry·Richard T PonYogesh S Sanghvi

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