Capturing Cell-Cell Interactions via SNAP-tag and CLIP-tag Technology

Bioconjugate Chemistry
S Hoehnel, Matthias P Lutolf


Juxtacrine or contact-dependent signaling is a major form of cell communication in multicellular organisms. The involved cell-cell and cell-extracellular-matrix (ECM) interactions are crucial for the organization and maintenance of tissue architecture and function. However, because cell-cell contacts are relatively weak, it is difficult to isolate interacting cells in their native state to study, for example, how specific cell types interact with others (e.g., stem cells with niche cells) or where they locate within tissues to execute specific tasks. To achieve this, we propose artificial in situ cell-to-cell linking systems that are based on SNAP-tag and CLIP-tag, engineered mutants of the human O6-alkylguanine-DNA alkyltransferase. Here we demonstrate that SNAP-tag can be utilized to efficiently and covalently tether cells to poly(ethylene glycol) (PEG)-based hydrogel surfaces that have been functionalized with the SNAP-tag substrate benzylguanine (BG). Furthermore, using PEG-based spherical microgels as an artificial cell model, we provide proof-of-principle for inducing clustering that mimics cell-cell pairing.


Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bita SahafLeonore A Herzenberg
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Antje KepplerKai Johnsson
Jul 23, 2004·Physiological Reviews·Gianfranco Bazzoni, Elisabetta Dejana
Apr 28, 2006·Protein Engineering, Design & Selection : PEDS·Thomas GronemeyerKai Johnsson
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Celeste M Nelson, Mina J Bissell
Mar 7, 2007·The Journal of Cell Biology·Lars E T JansenDon W Cleveland
Feb 23, 2008·Chemistry & Biology·Arnaud GautierKai Johnsson
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jennifer A QuinnHenry S Friedman
Apr 8, 2009·Journal of the American Chemical Society·Matthias A BrunKai Johnsson
Mar 12, 2010·ACS Chemical Biology·Damien MaurelKai Johnsson
May 21, 2010·Journal of Visualized Experiments : JoVE·Christopher R Provost, Luo Sun
Aug 17, 2010·Nature Medicine·Matthias T StephanDarrell J Irvine
Dec 31, 2010·Nature Methods·Teresa KleinMarkus Sauer
Jun 28, 2011·Chemistry & Biology·Karolina BojkowskaDidier Trono
Oct 11, 2011·Nature Methods·Samy GobaaMatthias P Lutolf
Sep 18, 2012·Current Opinion in Cell Biology·Richard L Klemke
Sep 26, 2013·Molecular and Cellular Biology·Harvey Lodish


Sep 10, 2020·Biomedicines·Fleury Augustin Nsole BitegheStefan Barth

Related Concepts

Cell Communication
Extracellular Matrix
Molecular Probe Techniques
Polyethylene Glycols
Stem Cells
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