Carbamate-linked cationic lipids with different hydrocarbon chains for gene delivery

Colloids and Surfaces. B, Biointerfaces
Jia ShiShubiao Zhang

Abstract

A series of carbamate-linked cationic lipids containing saturated or unsaturated hydrocarbon chains and quaternary ammonium head were designed and synthesized. After recrystallization, carbamate-linked cationic lipids with high purity (over 95%) were obtained. The structures of these lipids were proved by IR spectrum, HR-ESI-MS, HPLC, (1)H NMR and (13)C NMR. The liposomes were prepared by using these cationic lipids and neutral lipid DOPE. Particle size and zeta-potential were studied to show that they were suitable for gene transfection. The DNA-bonding ability of C12:0, C14:0 and C18:1 cationic liposomes was much better than others. The results of transfection showed that hydrophobic chains of these lipids have great effects on their transfection activity. The lipids bearing C12:0, C14:0 saturated chains or C18:1 unsaturated chain showed relatively higher transfection efficiency and lower cytotoxicity. So these cationic lipids could be used as non-viral gene carriers for further studies.

References

Jun 1, 2000·Biochimica Et Biophysica Acta·Y Perrie, G Gregoriadis
Aug 25, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangBaichao Ma
Apr 29, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Junjie LiZhishen Ge

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Citations

May 16, 2017·Gene Therapy·C ZylberbergS Matosevic
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiman BaiWei Huang
Dec 29, 2020·Chemistry and Physics of Lipids·Federica PontiGabriele Candiani
Jan 11, 2022·Chembiochem : a European Journal of Chemical Biology·Chopaka ThongbamrerBoon-Ek Yingyongnarongkul

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