Carbamazepine Attenuates Astroglial L-Glutamate Release Induced by Pro-Inflammatory Cytokines via Chronically Activation of Adenosine A2A Receptor

International Journal of Molecular Sciences
Motohiro OkadaYuto Ueda

Abstract

Carbamazepine (CBZ) binds adenosine receptors, but detailed effects of CBZ on astroglial transmission associated with adenosine receptor still need to be clarified. To clarify adenosinergic action of CBZ on astroglial transmission, primary cultured astrocytes were acutely or chronically treated with CBZ, proinflammatory cytokines (interferon γ (IFNγ) and tumor necrosis factor α (TNFα)), and adenosine A2A receptor (A2AR) agonist (CGS21680). IFNγ and TNFα increased basal, adenophostin-A (AdA)-evoked, and 2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (AMPA)-evoked astroglial L-glutamate releases. In physiological condition, CGS21680 increased basal astroglial L-glutamate release but glutamate transporter inhibition prevented this CGS21680 action. CBZ did not affect basal release, whereas glutamate transporter inhibition generated CBZ-induced glutamate release. Furthermore, AdA-evoked and AMPA-evoked releases were inhibited by CBZ but were unaffected by CGS21680. Contrary to physiological condition, chronic administrations of IFNγ and TNFα enhanced basal, AdA-, and AMPA-evoked releases, whereas IFNγ and TNFα decreased and increased CGS21680-evoked releases via modulation A2AR expression. Both chronic administration of ...Continue Reading

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Citations

Mar 19, 2020·International Journal of Molecular Sciences·Francisco Ciruela
Oct 1, 2020·Cells·Yoo Sung KimBo-Eun Yoon
Jul 8, 2020·Cellular and Molecular Neurobiology·Yingjiao LiuNai-Hong Chen
Nov 5, 2020·International Journal of Molecular Sciences·Kouji Fukuyama, Motohiro Okada
Jan 6, 2021·International Journal of Molecular Sciences·Motohiro OkadaTakashi Shiroyama
Mar 12, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Motohiro OkadaYuto Ueda
Mar 11, 2021·British Journal of Pharmacology·Motohiro Okada
Jun 3, 2021·International Journal of Molecular Sciences·Kouji Fukuyama, Motohiro Okada
Aug 26, 2021·Neuropathology and Applied Neurobiology·Ana Vasconcelos-FerreiraLuís Pereira de Almeida

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