PMID: 9186275May 1, 1997Paper

Carbamoylcholine regulation of polyphosphoinositide synthesis and hydrolysis in cultured, dispersed, digitonin-permeabilized mouse pancreatic islet cells

European Journal of Endocrinology
A M KardaszC J Hedeskov

Abstract

Continuing formation of inositol phosphates during stimulation of pancreatic beta-cells by hormones and neurotransmitters requires the continued synthesis of the polyphosphoinositides phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5 bisphosphate (PIP2) from phosphatidylinositol (PI). In the present study we have investigated how this pathway and the activity of phosphoinositide-specific phospholipase C (PI-PLC) are regulated by carbamoylcholine (CCh), Ca2+, the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), GTP gamma S and NaF in 44-h [3H]inositol-labelled, dispersed and digitonin-permeabilized mouse pancreatic islet cells. CCh stimulated not only PI-PLC (G-protein-mediated) but also, by an as yet unknown mechanism, significantly enhanced PI 4-kinase activity, estimated as the PIP:PI ratio, by 100%, and further increased the flux from PI to PIP and PIP2, GTP gamma S and NaF mimicked the effects of CCh on PI-PLC but had no effect on the levels of PIP and PIP2, TPA raised the PIP:PI ratio by 75%. In addition TPA counteracted the CCh stimulation of PI-PLC. There was no effect of 10(-6) mol/l Ca2+ on the levels of PIP and PIP2. Experiments with quinacrine and adenosine confirmed that PI-PLC and PI 4-ki...Continue Reading

Citations

Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Sarri, E Claro
Jan 16, 2008·Advances in Enzyme Regulation·Per-Olof Berggren, Christopher J Barker
Aug 8, 2007·The Biochemical Journal·Kylie M Wagstaff, David A Jans
Jul 17, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·G FridkinA Loyter

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