Carbamyl phosphate synthetase III, an evolutionary intermediate in the transition between glutamine-dependent and ammonia-dependent carbamyl phosphate synthetases

Journal of Molecular Biology
J HongP M Anderson

Abstract

The amino acid sequence of carbamyl phosphate synthetase (CPS) III from liver of spiny dogfish shark Squalus acanthias was deduced from the nucleotide sequence of its cDNA. Alignment of the derived amino acid sequence of CPS III with sequences of rat and frog CPS I and hamster CPS II reveals a high degree of amino acid identity, indicating that CPS III shares the same common ancestral genes as CPSs I and II. All of the CPSs examined show a high conservation of sequences in the adenine nucleotide binding domains and in residues that have been implicated in catalysis. The active-site cysteine residue required for glutamine-dependent activity by CPS II is preserved in the sequence of CPS III. Nevertheless, analysis of the protein sequences indicates that CPS III is more closely related to CPS I than to CPS II. The structure of CPS III, which is composed of a single polypeptide, is consistent with the view that CPS III evolved by fusion of separate genes coding for the glutaminase and synthetase domains of the enzyme and, like other CPSs, the synthetase domain evolved by duplication and fusion of an ancestral kinase gene. These results, together with the recent finding that frog CPS I retains the active site cysteine residue in the...Continue Reading

Citations

Oct 5, 2010·The Journal of Biological Chemistry·Christina C SpevakMatthew S Sachs
Jan 24, 2012·Journal of Experimental Botany·Judit PrihodaChris Bowler
Apr 7, 1998·JPEN. Journal of Parenteral and Enteral Nutrition·R McCauleyJ Hall
Apr 3, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Timothy E LindleyPaul M Anderson
Dec 1, 1996·Journal of Molecular Evolution·J HongP M Anderson
Jun 28, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yuen K IpShit F Chew
Jul 26, 1996·The Journal of Biological Chemistry·C R McCudden, S G Powers-Lee
Jun 7, 1996·The Journal of Biological Chemistry·H I Guy, D R Evans
Nov 19, 2020·Royal Society Open Science·Lewis J WhiteMary E Pownall
Jul 21, 2009·The Journal of Experimental Biology·Tammy LabergeM Danielle McDonald
Mar 21, 1998·Fungal Genetics and Biology : FG & B·J M Baek, C M Kenerley
May 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C P JenkinsonS D Cederbaum
Mar 17, 2004·Molecular Phylogenetics and Evolution·John K Moulton, Brian M Wiegmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.