Carbamylated erythropoietin mediates retinal neuroprotection in streptozotocin-induced early-stage diabetic rats

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Xiaojing LiuXun Xu

Abstract

The neuroprotective effect of carbamylated erythropoietin (CEPO), an erythropoietin (EPO) derivative, in diabetic retinopathy (DR) has not been clearly verified. We conducted this study to investigate the potential neuroprotective role of CEPO in a streptozotocin-induced diabetic rat model. Streptozotocin-induced diabetic rats and blank controls were treated with or without CEPO and EPO for 4 weeks. Retinal functional and histological changes were quantified by electroretinogram, light microscopy, and terminal dUTP nick end labeling assay. Gene and protein levels of colony-stimulating factor 2 receptor beta, low-affinity (CD131), EPO receptor (EPOR), THY1, glial fibrillary acidic protein (GFAP), and vascular endothelial growth factor (VEGF-A) in retinal tissues were determined by real-time PCR and western blotting, respectively. Vascular penetration was assessed by fluorescein retinal angiography. Diabetic rats had decreased retinal thickness, decreased ganglion cells, and increased retinal neuron apoptosis. CEPO increased CD131 and THY1 expression, while EPO increased EPOR expression. High glucose increased GFAP expression in the diabetic group, but both CEPO and EPO attenuated the trend for increase. CEPO downregulated VEGF-A...Continue Reading

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Citations

Apr 13, 2019·International Journal of Molecular Sciences·Shirley Suet Lee DingPooi Ling Mok
Nov 2, 2017·Neural Regeneration Research·Carolina Castillo HernándezJorge Fuentealba
Dec 4, 2019·Frontiers in Neuroscience·Maria Grazia RossinoGiovanni Casini
Feb 26, 2020·The American Journal of Pathology·Colin A BretzM Elizabeth Hartnett

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