PMID: 1214288Dec 4, 1975Paper

Carbamylcholine and acetylcholine-sensitive, cation-selective ionophore as part of the purified acetylcholine receptor

The Journal of Membrane Biology
A E Shamoo, M E Eldefrawi

Abstract

Black lipid membranes were formed with oxidized cholesterol in the presence of either the acetylcholine receptor, purified from the electric organ of the electric ray Torpedo californica or its tryptic digest. In both cases, conductance of cations increased and was dependent on the concentration of the receptor protein. Conductance of Ca++ was dependent on the concentration, but addition of carbamylcholine gave no reproducible of consistent effects. Only in the case of the tryptic digest of the acetylcholine receptor did carbamylcholine and acetylcholine consistently induce monovalent cation selective conductance (PNa,K: PCl=4.4). The induced monovalent cationic conductance due to carbamylcholine (10 muM) varied from 10- to over 100-fold. Curare (10muM) prevented the action of carbamylcholine. Na-dodecyl sulfate gel electrophoresis of the acetylcholine receptor, before and after tryptic digestion, indicated that this mild enzyme treatment hydrolyzed the receptor molecule subunits. Nevertheless, the receptor molecule retained its full binding of [acetyl(-3)H]acetylcholine; and analytical gel electrophoresis indicated that it remained intact possibly through hydrogen, hydrophobic and disulfice bonding.

References

Jan 1, 1976·Annals of the New York Academy of Sciences·D B SandersE E Cobb
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A E Shamoo, D H MacLennan
Jan 1, 1974·Annals of the New York Academy of Sciences·A E Shamoo
Jan 1, 1971·The Journal of General Physiology·F Ruiz-Manresa, H Grundfest
Sep 1, 1974·Archives of Biochemistry and Biophysics·M K Jain
Dec 1, 1974·Archives of Biochemistry and Biophysics·D MichaelsonM A Raftery
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E NeumannA Katchalsky
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A E Shamoo, R W Albers
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A Karlin, D Cowburn
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G L Hazelbauer, J P Changeux
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H W Chang
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M Michaelson, M A Raftery
Dec 10, 1973·Biochemical and Biophysical Research Communications·R C CarrollS J Edelstein
Dec 1, 1973·Biochemical Pharmacology·M E Eldefrawi, A T Eldefrawi
Sep 18, 1973·Biochemical and Biophysical Research Communications·G KempC E Wenner
Nov 1, 1973·Archives of Biochemistry and Biophysics·M E Eldefrawi, A T Eldefrawi
Mar 15, 1972·Experientia·W Leuzinger, M Schneider
Aug 1, 1981·Experimental Neurology·A DesoukiM E Eldefrawi
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Dec 29, 1977·Molecular and Cellular Biochemistry·F Hucho, W Schiebler
Dec 1, 1976·The Journal of Membrane Biology·C Miller, E Racker
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A T EldefrawiB Witkop
Jun 1, 1980·The Journal of Cell Biology·M W KlymkowskyR M Stroud
Jul 27, 1979·Biochemical and Biophysical Research Communications·D Bartfeld, S Fuchs
Jan 1, 1976·Annals of the New York Academy of Sciences·D B SandersE E Cobb
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·F J Barrantes
Jan 19, 1978·Biochimica Et Biophysica Acta·T WiedmerB W Fulpius
May 31, 1977·Biochimica Et Biophysica Acta·A E Shamoo, D A Goldstein

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