Carbaryl induces CYP1A1 gene expression in HepG2 and HaCaT cells but is not a ligand of the human hepatic Ah receptor

Toxicology and Applied Pharmacology
N LediracR Rahmani

Abstract

In spite of increasing numbers of insecticides used in agriculture, there are serious concerns regarding the potential risks of exposure to these agents. Carbaryl is one of the most important carbamate insecticides and has been used for about 30 years to control a wide range of pests. The study was designed to investigate if, among various insecticides currently used in world agriculture, this compound could induce human CYP1A1, an enzyme known to play an important role in the chemical activation of xenobiotics to genotoxic derivatives. Studies on HepG2 and HaCaT cell lines showed that carbaryl is capable of increasing, in a dose-dependent manner, both the ethoxyresorufin rufin-O-dec, O-deethylase activity and the steady-state concentrations of CYP1A1 mRNA, suggesting a transcriptional activation of this gene. When alpha-naphthoflavone, a partial Ah receptor (AhR) antagonist, and 8-methoxypsoralen, which interferes with the binding of activated AhR to the xenobiotic responsive element (XRE), were added to the cultures, CYP1A1 induction was suppressed. However, competitive binding studies using the 9S enriched fraction of human cytosol indicated that carbaryl did not displace [3H]TCDD from AhR. These data, together with the acti...Continue Reading

References

Aug 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·T IkedaY Shirasu
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Aug 15, 1994·Biochemical and Biophysical Research Communications·L AixP Galtier
Aug 4, 1995·The Journal of Biological Chemistry·M S Denison, J P Whitlock
Mar 28, 1995·Biochemical and Biophysical Research Communications·H G JeongK H Yang
Apr 17, 1995·Biochemical and Biophysical Research Communications·P LescaL Hoogenboom
Apr 17, 1995·Biochemical and Biophysical Research Communications·R WannerT Rosenbach
Jul 1, 1994·Trends in Pharmacological Sciences·A B OkeyP A Harper
Aug 1, 1994·Pharmacogenetics·Y TakahashiT Kamataki
Oct 1, 1993·Pharmacogenetics·H I Swanson, C A Bradfield
Feb 1, 1993·Pharmacogenetics·F J GonzalezM Yano
Nov 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M D ToddS B Farr

❮ Previous
Next ❯

Citations

Nov 1, 2011·Ecotoxicology·Bryan W Clark, Richard T Di Giulio
Dec 14, 2007·Chemical Research in Toxicology·Linh P Nguyen, Christopher A Bradfield
Apr 14, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jessica E Bohonowych, Michael S Denison
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Michael S Denison, Scott R Nagy
Jan 1, 2008·Drug Metabolism and Drug Interactions·Parikshit C DasErnest Hodgson
Jun 13, 2012·Journal of Toxicology and Environmental Health. Part a·Michaela Kalmes, Brunhilde Blömeke
May 9, 2002·Drug Metabolism Reviews·Slobodan Rendic
Dec 7, 2007·Drug Metabolism Reviews·Franz OeschRobert Landsiedel
Aug 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M BabínJ M Navas
Jun 6, 2016·Ecotoxicology and Environmental Safety·Diana MaierRita Triebskorn
Nov 7, 2006·Chemico-biological Interactions·Parikshit C DasRandy L Rose
Apr 8, 2018·Mucosal Immunology·Bruno LamasHarry Sokol
May 16, 2007·Journal of Cellular Physiology·Sebastien AntherieuRoger Rahmani
May 31, 2007·International Journal of Cancer. Journal International Du Cancer·Rajeev MahajanMichael C R Alavanja
May 13, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nathalie LediracRoger Rahmani
Jun 22, 2000·Archives of Biochemistry and Biophysics·M M IbaA B Okey
Jan 6, 2000·Archives of Biochemistry and Biophysics·C SandozA Carpy
Dec 16, 1998·Toxicology and Applied Pharmacology·M S DenisonM H Ziccardi
Nov 25, 2000·Toxicology·C DelescluseR Rahmani
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M FerrarisE Chiesara
Oct 27, 2004·Chemico-biological Interactions·Hollie I Swanson
Sep 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Asumi JinnoShoichi Fujita
Apr 20, 2001·Journal of Agricultural and Food Chemistry·A AbadA Montoya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.