Carbodiimide inactivation of MMPs and effect on dentin bonding

Journal of Dental Research
A MazzoniL Breschi

Abstract

The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demon...Continue Reading

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Citations

Jan 8, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Zihou ZhangDwayne Arola
Jan 9, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Gianluca TurcoLorenzo Breschi
Jan 9, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Andrea FrassettoMilena Cadenaro
Jan 15, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Milena CadenaroLorenzo Breschi
Jan 24, 2015·Operative Dentistry·L Tjäderhane
Jan 23, 2016·Operative Dentistry·F ShafieiM Mohammadi-Bassir
Dec 24, 2014·Journal of Dental Research·A MazzoniL Breschi
May 13, 2017·Journal of Dental Research·A MazzoniL Breschi
Dec 28, 2017·European Journal of Dentistry·Fereshteh ShafieiMoona Zamanpour
Jul 12, 2020·International Journal of Environmental Research and Public Health·Vittorio ChecchiAnnalisa Mazzoni
Sep 10, 2020·Brazilian Dental Journal·Beatriz Serrato CoelhoFlares Baratto-Filho
Oct 24, 2017·Calcified Tissue International·J CaiM F Burrow
Nov 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Eliseu A MünchowMarco C Bottino
Jul 17, 2020·Journal of Dentistry·Sandra Ribeiro CunhaLorenzo Breschi
Feb 19, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Paula A BaldionDiego E Betancourt
Mar 9, 2021·Advances in Pharmacological and Pharmaceutical Sciences·Eliane Avany Malveira AraújoMaria Fulgência Costa Lima Bandeira
Sep 26, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Zihou ZhangDwayne Arola
Jun 5, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Zihou ZhangDwayne Arola
Nov 29, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Annalisa MazzoniLorenzo Breschi
May 20, 2021·Journal of Dental Research·T MaravicA Mazzoni
Sep 19, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Zhongni ZhangCui Huang
Dec 18, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Milena CadenaroLorenzo Breschi
Apr 13, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Lívia Tosi TrevelinAna K Bedran-Russo
Jul 25, 2021·Materials·Tatjana MaravicAnnalisa Mazzoni
Aug 28, 2021·International Journal of Molecular Sciences·Tatjana MaravićAnnalisa Mazzoni
Sep 2, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Wendy ChenQuan-Li Li

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Methods Mentioned

BETA
electrophoresis
zymography

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