Carbohydrate and glycoprotein specificity of two endogenous cerebellar lectins

Biochimie
P MarschalJ P Zanetta

Abstract

Two endogenous cerebellar mannose binding lectins have been isolated in an active form by immunoaffinity chromatography employing their respective immobilized antibodies. One of them, termed cerebellar soluble lectin (CSL), was extracted in the absence of detergents, whereas the other, called Receptor 1 (R1), was soluble only in the presence of detergents. Tests of inhibition of agglutination of erythrocytes were performed with mono-, oligo and polysaccharides, as well as glycoconjugates of known structures. On the basis of agglutinating activities these 2 lectins are different from the previously reported lectins in brain, since they were not inhibited by galactosides and lactosides and were only marginally inhibited by glycosaminoglycans. CSL and R1 were better inhibited by mannose-rich glycopeptides as compared to the corresponding oligosaccharides. The different inhibition patterns obtained with glycans of known structures indicated that these lectins are very discriminative. Although CSL and R1 have similar specificities, they differed in their binding properties towards glycopeptides of ovalbumin. Both lectins showed considerable affinity for endogenous cerebellar glycopeptides, also rich in mannose. These glycopeptides b...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A KaplanW S Sly
Jan 1, 1979·Annual Review of Biochemistry·W Frazier, L Glaser
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1978·The Journal of Cell Biology·F GremoS H Barondes
Mar 24, 1977·Nature·D L SimpsonH H Loh
Jan 1, 1986·Annual Review of Cell Biology·G M Edelman
Dec 24, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H SchachterI Brockhausen
Feb 1, 1984·Journal of Neurochemistry·J P ZanettaG Vincendon
May 1, 1981·Biological Reviews of the Cambridge Philosophical Society·D R Garrod, A Nicol
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Sep 8, 1980·FEBS Letters·G I FranklinE J Thompson

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Citations

Jul 1, 1991·The Histochemical Journal·H J GabiusS Gabius
Nov 1, 1992·The Histochemical Journal·J P ZanettaG Vincendon
Jun 23, 1990·Lancet·J P ZanettaG Vincendon
Jan 1, 1993·Brain Research Bulletin·S LehmannJ P Zanetta
Sep 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·J S RodriguesR Gref
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S LehmannJ P Zanetta
Sep 13, 2013·Neurochemical Research·Filipe Marques GonçalvesRodrigo Bainy Leal
Aug 15, 1991·Archives of Biochemistry and Biophysics·F Y Zeng, H J Gabius

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