Carbohydrate and peptide structure of the alpha- and beta-subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma

Endocrine
M M ElliottL A Cole

Abstract

Human chorionic gonadotropin (hCG), purified from the urine of 14 individuals with normal pregnancy, diabetic pregnancy, hydatidiform mole, or choriocarcinoma, plus two hCG standard preparations, was examined for concurrent peptide-sequence and asparagine (N)- and serine (O)-linked carbohydrate heterogeneity. Protein-sequence analysis was used to measure amino-terminal heterogeneity and the "nicking" of internal peptide bonds. The use of high-pH anion-exchange chromatography coupled with the increased sensitivity of pulsed amperometric detection (HPAE/PAD) revealed that distinct proportions of both hCG alpha- and beta-subunits from normal and aberrant pregnancy are hyperglycosylated, and that it is the extent of the specific subunit hyperglycosylation that significantly increases in malignant disease. Peptide-bond nicking was restricted to a single linkage (beta 47-48) in normal and diabetic pregnancy, but occurred at two sites in standard preparations, at three sites in hydatidiform mole, and at three sites in choriocarcinoma beta-subunit. In the carbohydrate moiety, alpha-subunit from normal pregnancy hCG contained nonfucosylated, mono- and biantennary N-linked structures (49.3 and 36.7%, means); fucosylated biantennary and t...Continue Reading

References

Jul 1, 1992·The Journal of Clinical Endocrinology and Metabolism·M C SkarulisD L Blithe
Nov 1, 1992·The Biochemical Journal·A Stockell Hartree, A G Renwick
Jun 1, 1990·Journal of Biochemistry·R SakakibaraM Ishiguro
Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J RyanH T Keutmann
Jul 29, 1988·Biochemical and Biophysical Research Communications·J M BidartD Bellet
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Harbour-McMenaminJ E Blalock
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M M MatzukI Boime
Oct 1, 1987·The Journal of Clinical Endocrinology and Metabolism·L A Cole
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Jun 1, 1994·The Journal of Clinical Endocrinology and Metabolism·L WideC Rasmussen

❮ Previous
Next ❯

Citations

Jan 13, 1999·Prenatal Diagnosis·J F O'ConnorG Kovalevskaya
Aug 17, 2011·Reviews in Endocrine & Metabolic Disorders·Prajna Banerjee, Asgerally T Fazleabas
Sep 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·P BergerUNKNOWN Members of the ISOBM TD-7 Workshop on hCG and Related Molecules
Feb 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jaime M Sutton
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Linda M ThienpontAndré P De Leenheer
Nov 26, 2003·Clinical Biochemistry·Laurence A ColeNicole D Stephens
Dec 26, 2001·Archives of Medical Research·A Ulloa-AguirreC Timossi
Sep 21, 2002·Molecular and Cellular Endocrinology·G KovalevskayaJ F O'Connor
Jul 10, 2003·Molecular and Cellular Endocrinology·S BirkenWilliam Moyle
Apr 14, 1999·Molecular Medicine Today·J A CanickH S Cuckle
Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E. I. Kohorn, L. Cole
Oct 24, 2008·Human Reproduction Update·S F de Medeiros, R J Norman
Sep 16, 2003·Clinical Obstetrics and Gynecology·Laurence A Cole, Jaime M Sutton
Jun 23, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Steven Birken
Mar 30, 2012·Reproductive Biology and Endocrinology : RB&E·Laurence A Cole
Jan 28, 2009·Reproductive Biology and Endocrinology : RB&E·Laurence A Cole
Dec 14, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Laurence A Cole
Mar 4, 2005·Treatments in Endocrinology·Roberto Lanes
Oct 3, 2014·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Laurence A Cole
Aug 20, 2015·Critical Reviews in Clinical Laboratory Sciences·Ulf-Håkan Stenman
Oct 13, 2009·Expert Review of Molecular Diagnostics·Laurence A Cole
Dec 23, 2008·Expert Review of Molecular Diagnostics·Laurence A Cole
Dec 18, 2015·American Journal of Reproductive Immunology : AJRI·Jemma Evans
Dec 1, 2008·Expert Opinion on Medical Diagnostics·P Berger, C Sturgeon
Nov 22, 2005·Journal of the American Pharmacists Association : JAPhA·Laurence A ColeWilliam F Rayburn
Feb 8, 2011·Placenta·W NorrisS Kalkunte
Jun 30, 2009·Journal of Reproductive Immunology·Laurence A Cole
May 30, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mark A CervinskiAnn M Gronowski
Nov 26, 2008·Clinical Biochemistry·Laurence A Cole, Sarah A Khanlian
Nov 15, 2008·Gynecologic Oncology·Carolyn Y Muller, Laurence A Cole
Apr 25, 2008·Fertility and Sterility·Laurence A ColeFrancis W Byrn
Aug 7, 2007·Fertility and Sterility·Yasushi SasakiLaurence A Cole
Mar 10, 2007·Placenta·L A Cole
Nov 29, 2005·Obstetrics and Gynecology Clinics of North America·Laurence A ColeHarriet O Smith
Apr 17, 2008·British Journal of Pharmacology·U-H StenmanH Alfthan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.