PMID: 7017728Apr 1, 1981Paper

Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated

Proceedings of the National Academy of Sciences of the United States of America
C HashimotoC E Ballou

Abstract

Carboxypeptidase Y, a vacuolar enzyme from Saccharomyces cerevisiae, was digested with endo-beta-N-acetyl-D-glucosaminidase H to release the four oligosaccharide chains that are linked to asparagine in the glycoprotein. The oligosaccharides were fractionated into a neutral and acidic component, and the latter proved to phosphorylated. From its gel filtration pattern, the neutral fraction was shown to be a mixture of at least four homologs, the smallest of which had a proton NMR spectrum almost identical to that given by an IgM oligosaccharide with eight mannoses and one N-acetylglucosamine [Cohen, R. E. & Ballou, C. E. (1980) Biochemistry 19, 4345--4358]. The yeast oligosaccharide has one additional mannose unit in an alpha 1 leads to 3 or alpha 1 leads to 6 linkage, whereas the larger homologs appear to have two, three, and four more mannose units. One phosphorylated oligosaccharides with a mannose/phosphate ratio of 12.5 was reduced with NaB3H4 and then subjected to mild acid hydrolysis. This released mannose and mannobiose that were glycosidically linked to the phosphate group, whereas complete acid hydrolysis yielded D-mannose 6-phosphate. The recovered oligosaccharide phosphomonoester, which contained 11 or 12 mannose unit...Continue Reading

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Citations

Jan 1, 1991·Folia Microbiologica·J Horák
Jun 1, 1990·Molecular and Biochemical Parasitology·C L JaffeL F Schnur
Apr 27, 1987·Biochimica Et Biophysica Acta·W Tanner, L Lehle
Jun 19, 1993·Biochimica Et Biophysica Acta·J RühlmannE Köttgen
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J Frevert, C E Ballou
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T C Huffaker, P W Robbins
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P K TsaiC E Ballou
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L BallouC E Ballou
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Nov 24, 1999·Glycobiology·B SchmauserR Tauber
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Jul 23, 2005·The Journal of Chemical Physics·Małgorzata LekkaLászló Forró
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