Carbohydrate composition of Carcinus aestuarii hemocyanin

Archives of Biochemistry and Biophysics
P Dolashka-AngelovaW Voelter

Abstract

The hemocyanin of the crab Carcinus aestuarii contains a carbohydrate moiety that represents 1.6% of protein mass. This carbohydrate content is higher than that exhibited by other arthropod hemocyanins so far investigated. By combination of FPLC ion exchange chromatography and reverse-phase HPLC, the native oligomeric protein can be resolved into three major and one minor electrophoretically pure fractions that are found to be homogeneous by N-terminal sequencing and correspond to the subunit polypeptide chains. Sugar analysis on the different subunits reveals that the subunit referred to as Ca2 is glycosylated, with a carbohydrate content of 6.3%. By Ca2 trypsin digestion, separation of glycopeptides, and amino acid sequencing, three consensus sequences for O-glycosylation and one for N-glycosylation were found. MALDI-MS was applied for the determination of the molecular masses of the various glycopeptides and peptides after removal of carbohydrates by neuraminidase and alpha-N-acetylgalactosaminidase.

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Citations

Mar 5, 2014·Chemical Reviews·Edward I SolomonLi Tian
Jun 11, 2011·Glycoconjugate Journal·Lyudmila VelkovaBart Devreese
Jul 25, 2012·Journal of Fluorescence·Elena KostadinovaIvan Petkov
Mar 16, 2013·Journal of Fluorescence·Elena KostadinovaWolfgang Voelter
Jun 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika Staudacher
Oct 31, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Pauline PinnowThorsten Burmester
Nov 18, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tingjun FanRishan Cong
Aug 11, 2015·Marine Biotechnology·A DolashkiP Dolashka
Apr 20, 2004·Biochemical and Biophysical Research Communications·Pavlina Dolashka-AngelovaWolfgang Voelter
Dec 17, 2014·Marine Biotechnology·Aleksandar DolashkiPavlina Dolashka

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