Sep 1, 1975

Carbohydrate containing media for the detection of urease in "Klebsiella"

Annales de microbiologie
C Richard

Abstract

Sone strains of Klebsiella pneumoniae and K. oxytoca grown on nutrient agar may appear "urease negative" in a Ferguson type reagent medium after a 24 h incubation at 37 degrees C. Amongst such 147 so called urease negative strains, urease has been detected within a few hours in 79 strains, when bacteria have grown on media containing carbohydrates (Kligler iron agar, Drigalski lactose agar, SS agar and Worfel-Ferguson sucrose medium). Acid production by carbohydrate fermentation increases urease production by Klebsiella: pH 4 is the most convenient pH for urease synthesis by these bacteria. The other 68 strains have been considered as urease-less Klebsiella. The best results are obtained from culture on Worfel-Ferguson sucrose medium: urea hydrolysis is positive--on an average-after 1 hour and 30 minutes when detected in a Ferguson type reagent medium, and after 2 hours and 35 minutes when detected in a Christensen reagent medium.

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Mentioned in this Paper

Carbohydrate nutrients
Agar
Klebsiella pneumoniae
Infection by Klebsiella Pneumoniae in Conditions Classified Elsewhere and of Unspecified Site
Bacteriological Techniques
Urea Measurement
Carbohydrate Metabolism
Urease
Klebsiella Infections
Phytourease

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