Carbohydrate-controlled precipitation of apatite with coprecipitation of organic molecules in human saliva: stabilizing role of polyols

Calcified Tissue International
K K MäkinenL M Park

Abstract

Addition of common dietary carbohydrates to Millipore-treated human whole saliva either enhances or inhibits the formation of salivary precipitates, some carbohydrates showing no effect. The purpose of this study was to investigate the precipitation conditions more thoroughly and to elucidate the chemical nature of the precipitates formed. D-Xylose either enhanced precipitation (in long-term incubations) or had no appreciable effect (in 10 minute incubations). Other aldo- and keto-sugars and disaccharides (maltose, sucrose, lactose) generally enhanced precipitation, whereas all polyols (xylitol, D-sorbitol, mannitol, and maltitol) retarded the formation of turbidity in saliva. Xylitol inhibited formation of precipitates also in the presence of D-xylose, dextrans, and starch. Fast protein liquid chromatography (FPLC) of EDTA-soluble pellets obtained by centrifugation of the precipitates produced two major protein fractions (I and II) with a molecular weight of 112,000 and 46,000, respectively. The carbohydrates exerted a selective effect on the relative size of I and II in that polyol incubations resulted in a I to II ratio of 1:3, whereas control incubations (without added sugars) and incubations with other carbohydrates gave r...Continue Reading

References

Aug 24, 1979·Calcified Tissue International·E C MorenoD I Hay
Oct 1, 1979·The Biochemical Journal·A BennickG Madapallimattam
Jan 1, 1976·Archives of Oral Biology·P Gron, D I Hay
Apr 1, 1986·Scandinavian Journal of Dental Research·E Söderling, K K Mäkinen
Dec 1, 1985·Acta Odontologica Scandinavica·J BánóczyA Scheinin
Jul 1, 1971·The Biochemical Journal·A Bennick, G E Connell
Jun 29, 1971·Biochimica Et Biophysica Acta·F A Bettelheim
Mar 1, 1982·Calcified Tissue International·G Williams, J D Sallis
Jan 1, 1984·Calcified Tissue International·K K Mäkinen, E Söderling
Apr 1, 1984·The Journal of the American Dental Association·W J LoescheR Corpron
Aug 1, 1983·Journal of Dental Research·C VadeboncoeurD Mayrand
Jan 1, 1984·Caries Research·J ArendsM T Smits
Jul 1, 1983·The British Journal of Nutrition·M M Hämäläinen, K K Mäkinen
Jan 1, 1981·Archives of Oral Biology·J M BorggrevenF C Driessens
Jul 1, 1981·Journal of Biochemistry·K Gekko, T Morikawa
Jul 1, 1981·Journal of Biochemistry·K Gekko, T Morikawa
Nov 11, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·I E Horgan

❮ Previous
Next ❯

Citations

Jan 1, 1993·Journal of American College Health : J of ACH·K K Mäkinen
Mar 27, 2010·International Journal of Dentistry·Kauko K Mäkinen
Aug 23, 2007·Macromolecular Bioscience·Sylvia Schweizer, Andreas Taubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.