Carbohydrate cytochemistry of the intestinal epithelium of Ascaris suum. Nature of the microvilli glycocalyx and basal lamella.

Zeitschrift Für Parasitenkunde
J J Trimble, S A Thompson

Abstract

Results of various cytochemical tests demonstrate large deposits of glycogen within the intestinal absorptive cells of Ascaris suum. Carbohydrate material is also associated with the microvilli surface and basal lamella. Staining produced by the periodate-thiocarbohydrazide-osmium procedure was abolished by analine or m-aminophenol. Diastase digestion did not alter the staining on the microvilli surface. Similar results were seen using the silver methenamine procedure. A positive reaction was noted on the microvilli surface, vesicles in both the apical and basal cytoplasm, Golgi apparatus, and basal lamella. Lanthanum nitrate stained the microvilli surface and intercellular spaces between absorptive cells. Alcian blue or cetylpyridinium chloride in combination with lanthanum enhanced the staining produced by lanthanum alone. These results suggest the presence of acidic glycans on both the microvilli surface and basal lamella.

References

Jan 1, 1966·The Anatomical Record·A RambourgC P Leblond
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jan 1, 1970·International Review of Cytology·R J Winzler
Nov 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M SeligmanL Katzoff
Jun 1, 1967·The Journal of Cell Biology·J P Revel, M J Karnovsky

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