Carbohydrate digestion in sandflies: alpha-glucosidase activity in the midgut of Phlebotomus langeroni

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
R J Dillon, E el-Kordy

Abstract

Midgut alpha-glucosidase (EC 3.2.1.20) activities were measured after ingestion of blood and sugar meals by the phlebotomine sandfly Phlebotomus langeroni. alpha-Glucosidase activity increased significantly within 1 hr after a blood meal and was maintained at significantly high activities until 48 hr postfeeding, when it fell to basal activity levels. Midgut alpha-glucosidase activity also increased within 1 hr of feeding on a sucrose meal, but there was no discernable peak in activity during the days postingestion. Differences in the induction of enzyme activity after a sugar meal compared to a blood meal might reflect the mode of ingestion of the two types of meal. The sugar meal is released intermittently into the midgut from the crop, in contrast to the bloodmeal, which is directed into the midgut immediately after ingestion and digested in a "batch" process. Nearly 90% of the alpha-glucosidase activity was associated with midgut cells of sugar fed sandflies compared to only 46% in blood fed insects. Isoelectrofocusing revealed the presence of seven alpha-glucosidases with isoelectric points between 4.3-5.8. No alpha-glucosidase activity was detected in the crop, indicating that glucosidases originate from the midgut epithe...Continue Reading

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Citations

Feb 12, 2013·Journal of Molecular Evolution·Marek Gabriško
Dec 25, 2012·Parasitology Research·Sriwatapron Sor-SuwanWej Choochote
Mar 23, 2000·Insect Biochemistry and Molecular Biology·J M RibeiroR Charlab
Sep 11, 2009·PloS One·Flávia Borges MuryMarilvia Dansa-Petretski
Jan 16, 2013·Annual Review of Entomology·John G Stoffolano, Aaron T Haselton
Jul 31, 2012·Journal of Insect Physiology·Vladimir F ValeNelder F Gontijo
Apr 24, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jayme A Souza-NetoPaulo E M Ribolla
May 16, 2006·Memórias do Instituto Oswaldo Cruz·Reginaldo R CavalcanteNelder de F Gontijo
Sep 22, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Doron RosenzweigDan Zilberstein
Feb 13, 2013·Insect Molecular Biology·J AbrudanM A McDowell
Nov 13, 2019·Journal of Insect Physiology·Denise Barguil NepomucenoNelder Figueiredo Gontijo

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