PMID: 16659573May 1, 1976Paper

Carbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus

Plant Physiology
W F Hunt, R S Loomis


Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 m sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0.61 +/- 0.04 gram dry tissue per gram sucrose supplied). Initial specific growth rates exhibited a Michaelis-Menten dependency on initial sucrose concentration such that the V(max) = 0.18 g g(-1) day(-1) and Km = 0.0037 m sucrose.


May 1, 1962·Plant Physiology·J Straus
Feb 1, 1969·Plant Physiology·J P HelgesonC D Upper
Jul 1, 1970·Plant Physiology·C D UpperG T Haberlach

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Dec 5, 1991·Biotechnology and Bioengineering·S R SchnappP M Hasegawa
Dec 1, 1978·Plant Physiology·S E HampsonD W Rains

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