Carbohydrate microarray for the detection of glycan-protein interactions using metal-enhanced fluorescence

Analytical Chemistry
Jie YangSabine Szunerits

Abstract

Carbohydrate arrays are potentially one of the most attractive tools to study carbohydrate-based interactions. This paper describes a new analytical platform that exploits metal-enhanced fluorescence for the sensitive and selective screening of carbohydrate-lectin interactions. The chip consists of a glass slide covered with gold nanostructures, postcoated with a thin layer of amorphous silicon-carbon alloy (a-Si0.8C0.2:H). An immobilization strategy based on the formation of a covalent bond between propargyl-terminated glycans and surface-linked azide groups was used to attach various glycans at varying surface densities onto the interface and to fabricate a carbohydrate array via efficient local "click" chemistry strategy. The specific association of the new interface with fluorescently labeled lectins was assessed by fluorescence imaging and an excellent selectivity to specific proteins was achieved. Optimization of the surface architecture and the plasmonic transducer resulted in an enhancement of the fluorescence intensity by 1 order of magnitude, when compared to the corresponding substrate devoid of gold nanostructures. The limit of detection (LOD) of such microarrays is in the picomolar range, making it a promising syst...Continue Reading

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Citations

Mar 18, 2016·Biosensors & Bioelectronics·Na Rae JoYong-Beom Shin
Mar 15, 2016·Analytical and Bioanalytical Chemistry·Christoph Haisch
Apr 19, 2016·Biosensors & Bioelectronics·Xiaofan JiJunxue Fu
May 26, 2017·Chemical Communications : Chem Comm·Moritz BuhlBart Jan Ravoo
Sep 10, 2020·Nanomaterials·Mohsin Ali BadshahMuhammad Wajid Saleem

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