Carbohydrate Syntrophy enhances the establishment of Bifidobacterium breve UCC2003 in the neonatal gut

Scientific Reports
Mary O'Connell MotherwayDouwe van Sinderen

Abstract

The non-digestible oligosaccharide fraction of maternal milk represents an important of carbohydrate and energy source for saccharolytic bifidobacteria in the gastrointestinal tract during early life. However, not all neonatal bifidobacteria isolates can directly metabolise the complex sialylated, fucosylated, sulphated and/or N-acetylglucosamine-containing oligosaccharide structures present in mothers milk. For some bifidobacterial strains, efficient carbohydrate syntrophy or crossfeeding is key to their establishment in the gut. In this study, we have adopted advanced functional genomic approaches to create single and double in-frame deletions of the N-acetyl glucosamine 6-phosphate deacetylase encoding genes, nagA1 and nagA2, of B. breve UCC2003. In vitro phenotypic analysis followed by in vivo studies on co-colonisation, mother to infant transmission, and evaluation of the relative co-establishment of B. bifidum and B. breve UCC2003 or UCC2003ΔnagA1ΔnagA2 in dam-reared neonatal mice demonstrates the importance of crossfeeding on sialic acid, fucose and N-acetylglucosamine-containing oligosaccharides for the establishment of B. breve UCC2003 in the neonatal gut. Furthermore, transcriptomic analysis of in vivo gene expression...Continue Reading

References

Dec 8, 1995·The Journal of Biological Chemistry·P A PrietoM Pierce
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Mar 26, 2003·Bioinformatics·Jorge García de la NavaAlberto Pascual-Montano
Nov 14, 2006·Applied and Environmental Microbiology·Alain MazéDouwe van Sinderen
Aug 21, 2007·Applied Microbiology and Biotechnology·Pablo Alvarez-MartínBaltasar Mayo
Oct 30, 2007·Applied and Environmental Microbiology·Michelle CroninDouwe van Sinderen
Aug 12, 2008·Applied and Environmental Microbiology·Mary O'Connell MotherwayDouwe van Sinderen
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·D A SelaD A Mills
Dec 31, 2008·Applied and Environmental Microbiology·Karina PokusaevaDouwe van Sinderen
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francesca TurroniMarco Ventura
May 1, 2009·Microbial Biotechnology·Mary O'Connell MotherwayDouwe Van Sinderen
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mary O'Connell MotherwayDouwe van Sinderen
Mar 19, 2014·The British Journal of Nutrition·Simone AlbrechtPauline M Rudd
May 13, 2014·Applied and Environmental Microbiology·Muireann EganDouwe van Sinderen
May 23, 2014·Annual Review of Nutrition·Jennifer T SmilowitzSamara L Freeman
Nov 12, 2014·Annual Review of Animal Biosciences·Alline R PachecoDavid A Mills
Jun 25, 2016·Nature Communications·Takahiro MatsukiKen Kurokawa
Oct 30, 2016·Applied and Environmental Microbiology·Muireann EganDouwe van Sinderen
Jun 24, 2017·Microbiology Spectrum·Claudio Hidalgo-CantabranaAbelardo Margolles

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Datasets Mentioned

BETA
GSE110077

Methods Mentioned

BETA
PCRs
PCR
Feature Extraction

Software Mentioned

Cyber
Agilent
Feature Extraction

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