Carbohydrate Utilization in Lactobacillus sake.

Applied and Environmental Microbiology
R LauretM Zagorec

Abstract

The ability of Lactobacillus sake to use various carbon sources was investigated. For this purpose we developed a chemically defined medium allowing growth of L. sake and some related lactobacilli. This medium was used to determine growth rates on various carbohydrates and some nutritional requirements of L. sake. Mutants resistant to 2-deoxy-d-glucose (a nonmetabolizable glucose analog) were isolated. One mutant unable to grow on mannose and one mutant deficient in growth on mannose, fructose, and sucrose were studied by determining growth characteristics and carbohydrate uptake and phosphorylation rates. We show here that sucrose, fructose, mannose, N-acetylglucosamine, and glucose are transported and phosphorylated by the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). The PTS permease specific for mannose, enzyme II(supMan), was shown to be responsible for mannose, glucose, and N-acetylglucosamine transport. A second, non-PTS system, which was responsible for glucose transport, was demonstrated. Subsequent glucose metabolism involved an ATP-dependent phosphorylation. Ribose and gluconate were transported by PTS-independent permeases.

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Citations

May 3, 2000·Journal of Applied Microbiology·M ElliL Morelli
Jun 19, 2001·FEMS Microbiology Letters·A MarceauM C Champomier-Vergès
Jul 31, 2002·Applied and Environmental Microbiology·Marie-Christine Champomier-VergèsMonique Zagorec
Oct 25, 2007·Journal of Molecular Microbiology and Biotechnology·Borja SánchezMonique Zagorec
Mar 9, 2010·Applied and Environmental Microbiology·Fabrizio ChiaramonteMonique Zagorec
Jan 22, 2013·Applied and Environmental Microbiology·Jamila Anba-MondoloniMarie-Christine Champomier-Vergès
Jul 16, 2003·Journal of Applied Microbiology·M PiuriS M Ruzal
Dec 3, 2004·Applied and Environmental Microbiology·Anika MarceauMarie-Christine Champomier-Vergès
Nov 11, 1999·FEMS Microbiology Letters·M C Champomier VergèsS D Ehrlich
Feb 8, 2002·Microbiology·Anne-Marie DudezMonique Zagorec
Jul 3, 2007·Applied and Environmental Microbiology·Yohann CoutéAbelardo Margolles
Dec 31, 2008·Applied and Environmental Microbiology·Stéphane ChaillouMonique Zagorec
Jul 19, 2020·Applied and Environmental Microbiology·Emilie VerplaetseMarie-Christine Champomier-Vergès
Oct 27, 2001·Applied and Environmental Microbiology·J Lee, H P Blaschek
Nov 12, 2014·Journal of Basic Microbiology·Hui-qing XuYu Chen
Aug 25, 2012·BMB Reports·Kyunghun JeongJaehan Kim
Feb 13, 2007·Current Microbiology·Kristine NaterstadLars Axelsson
Sep 8, 2017·Microorganisms·Monique Zagorec, Marie-Christine Champomier-Vergès
Oct 5, 2017·Canadian Journal of Microbiology·Jordyn Bergsveinson, Barry Ziola
Sep 14, 2018·Frontiers in Microbiology·Chiara MontanariGiulia Tabanelli
Jun 1, 1997·Applied and Environmental Microbiology·R StentzM Zagorec
Jun 9, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Yi-Chao ChenFa-Jui Tan
Mar 6, 2002·Systematic and Applied Microbiology·L PartanenT Alatossava
Mar 14, 2012·BMC Microbiology·Solveig SchmidAnne-Marie Crutz-Le Coq

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