Abstract
The non-invasive technique of 13C-nuclear magnetic resonance was applied to study glucose metabolism in vivo in Trypanosoma cruzi, the causative agent of American trypanosomiasis (Chagas' disease). It was found that under anaerobic conditions [1-13C]glucose undergoes a glycolytic pathway whose main metabolic products were identified as [3-13C]alanine, [2-13C]succinate and phosphoryl[1-13C]choline; [2-13C]alanine was also a minor metabolite. The addition of 70% 2H2O to the incubation mixture led to the formation of [3-13C, 3-2H]alanine derived from the prior incorporation of 2H+ into pyruvate. The existence of a [3-13C, 3-2H]pyruvate precursor, although not isolated, could be inferred from the formation of [2-13C, 2-2H]succinate in the same experiment. The latter derives from the CO2 fixation reaction on pyruvate or phosphoenolpyruvate to give malate, which is then converted to succinate through the fumarate intermediate step. The presence of [2-13C]alanine must be traced to a randomization of label at the malate-fumarate stage. Both [3-13C, 3-2H]alanine and [2-13C, 2-2H]succinate were excreted from the cells into the supernatant. When the cell pellet was lysed with perchloric acid it released [3-13C]alanine which was devoid of ...Continue Reading
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