PMID: 2120054Sep 11, 1990Paper

Carbon-13 nuclear magnetic resonance analysis of [1-13C]glucose metabolism in Trypanosoma cruzi. Evidence of the presence of two alanine pools and of two CO2 fixation reactions

European Journal of Biochemistry
B FrydmanJ J Cazzulo

Abstract

The non-invasive technique of 13C-nuclear magnetic resonance was applied to study glucose metabolism in vivo in Trypanosoma cruzi, the causative agent of American trypanosomiasis (Chagas' disease). It was found that under anaerobic conditions [1-13C]glucose undergoes a glycolytic pathway whose main metabolic products were identified as [3-13C]alanine, [2-13C]succinate and phosphoryl[1-13C]choline; [2-13C]alanine was also a minor metabolite. The addition of 70% 2H2O to the incubation mixture led to the formation of [3-13C, 3-2H]alanine derived from the prior incorporation of 2H+ into pyruvate. The existence of a [3-13C, 3-2H]pyruvate precursor, although not isolated, could be inferred from the formation of [2-13C, 2-2H]succinate in the same experiment. The latter derives from the CO2 fixation reaction on pyruvate or phosphoenolpyruvate to give malate, which is then converted to succinate through the fumarate intermediate step. The presence of [2-13C]alanine must be traced to a randomization of label at the malate-fumarate stage. Both [3-13C, 3-2H]alanine and [2-13C, 2-2H]succinate were excreted from the cells into the supernatant. When the cell pellet was lysed with perchloric acid it released [3-13C]alanine which was devoid of ...Continue Reading

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Citations

Apr 1, 1994·Journal of Bioenergetics and Biomembranes·J J Cazzulo
Oct 1, 1994·Molecular and Biochemical Parasitology·F ChaumontF R Opperdoes
Oct 8, 1999·Molecular and Biochemical Parasitology·J J BlumL Vieira
Oct 17, 2006·Parasitology Today·A G Tielens, J J Van Hellemond
Mar 16, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R A Webb, L Xue
Jan 29, 2000·European Journal of Biochemistry·D G CapellutoJ J Cannata
May 19, 2009·BMC Systems Biology·Seth B RobertsGregory A Buck
Apr 1, 1994·Journal of Bioenergetics and Biomembranes·J J Blum
Mar 3, 2007·The Journal of Biological Chemistry·Carlos E Sanz-RodríguezJulio A Urbina
Oct 26, 2011·Essays in Biochemistry·Dante A MaugeriJuan-José Cazzulo
Jun 23, 2015·Electrophoresis·Gisele A B CanutoMarina F M Tavares
Jul 20, 2018·MSphere·Richard M B M GirardAriel Mariano Silber
Oct 7, 2020·PLoS Neglected Tropical Diseases·Isabel S ShiratsubakiJair L Siqueira-Neto
Aug 18, 2020·International Journal of Biological Macromolecules·Santanu Sasidharan, Prakash Saudagar
Sep 28, 1998·Biochimica Et Biophysica Acta·L L Vieira
Jan 1, 1994·Parasitology Today·J A Urbina

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