PMID: 6980672Jun 8, 1982Paper

Carbon-13 nuclear magnetic resonance studies of acetate metabolism in intact cells of rhodopseudomonas sphaeroides

Biochimica Et Biophysica Acta
K NicolayW N Konings

Abstract

13C-nuclear magnetic resonance was used to study the metabolism of [2-(13)C]acetate in suspensions of Rhodopseudomonas sphaeroides. In the dark, in logarithmic-phase cells the 13C label appeared first in butyrate C-2 and C-4 and subsequently in glutamate C-4 and succinate C-2 and C-3. In the light, synthesis of poly(beta-hydroxybutyrate) (PHB) takes place. Butyrate synthesis seems to be independent of PHB synthesis or degradation activity. Starved, logarithmic-phase cells also show massive synthesis of PHB in the dark. Stationary-phase cells incorporate 13C predominantly into glutamate and succinate. No significant butyrate biosynthesis can be detected in the dark or during illumination. The incorporation of label in PHB is very slow in these cells and most probably originates from exchange of 12C for 13C into PHB. This might indicate slow turnover without net synthesis of the polymer occurring under these conditions. The results are discussed in relation to the redox state and the availability of metabolic energy for biosynthetic reactions in the dark and during illumination of cell suspensions of Rps. sphaeroides.

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A den HollanderR G Shulman
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S M CohenR G Shulman
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S M CohenA C McLaughlin
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UgurbilR G Shulman
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A den HollanderR G Shulman
Jan 1, 1981·Annual Review of Biochemistry·D G Gadian, G K Radda
Aug 17, 1981·FEBS Letters·D D DoyleM Bárány
Jun 1, 1960·Journal of General Microbiology·W R SISTROM

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Citations

Jul 22, 2006·Molecular Microbiology·Birgit E AlberGeorg Fuchs
Dec 15, 1982·Biochemical and Biophysical Research Communications·K M BrindleN Soffe

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