Carbon Allocation in Rhodococcus jostii RHA1 in Response to Disruption and Overexpression of nlpR Regulatory Gene, Based on (13)C-labeling Analysis

Frontiers in Microbiology
Martín A HernándezHéctor M Alvarez

Abstract

Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of (13)C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher (13)C abundance (∼60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also promoted a decrease in the label incorporation into several TLE-derivative fractions including neutral lipids (NL), glycolipids (GL), phospholipids (PL), triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids (FFA), with the DAG being the most affected. In contrast, the nlpR overexpression in RHA1 cells under nitrogen-rich conditions produced an increase of the label incorporation into the TLE and its derivative NL and PL fractions, the last one being the highest (13)C e...Continue Reading

References

Sep 1, 2000·Applied Microbiology and Biotechnology·H M AlvarezA Steinbüchel
Jun 21, 2001·Applied Microbiology and Biotechnology·I Voss, A Steinbüchel
Sep 1, 1995·Applied and Environmental Microbiology·M SetoK Yano
Aug 3, 2007·Carbohydrate Research·Malcolm B PerryEvgeny Vinogradov
Jun 12, 2008·Histochemistry and Cell Biology·Toyoshi FujimotoYuki Shinohara
Jul 16, 2009·Rapid Communications in Mass Spectrometry : RCM·Andreas RichterGerd Gleixner
May 7, 2013·BMC Genomics·Victoria A JenkinsKerstin J Williams
Oct 29, 2013·Applied and Environmental Microbiology·Sandra M HeinzelmannMarcel T J van der Meer
Jun 17, 2014·Applied Microbiology and Biotechnology·Li-Li YaoBang-Ce Ye
Jun 17, 2015·Molecular Microbiology·Kerstin J WilliamsBrian D Robertson
Dec 9, 2015·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Heng LiaoBang-Ce Ye

❮ Previous
Next ❯

Citations

Jul 22, 2019·Applied and Environmental Microbiology·Héctor M AlvarezMaria S Villalba
Jan 31, 2021·Biotechnology Advances·Miroslav PátekJan Nešvera
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Héctor M AlvarezMaría S Villalba

❮ Previous
Next ❯

Methods Mentioned

BETA
column chromatography
Optic microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.