Carbon Assimilation Strategies in Ultrabasic Groundwater: Clues from the Integrated Study of a Serpentinization-Influenced Aquifer

MSystems
Lauren Marie SeylerMatthew O Schrenk

Abstract

Serpentinization is a low-temperature metamorphic process by which ultramafic rock chemically reacts with water. Such reactions provide energy and materials that may be harnessed by chemosynthetic microbial communities at hydrothermal springs and in the subsurface. However, the biogeochemistry mediated by microbial populations that inhabit these environments is understudied and complicated by overlapping biotic and abiotic processes. We applied metagenomics, metatranscriptomics, and untargeted metabolomics techniques to environmental samples taken from the Coast Range Ophiolite Microbial Observatory (CROMO), a subsurface observatory consisting of 12 wells drilled into the ultramafic and serpentinite mélange of the Coast Range Ophiolite in California. Using a combination of DNA and RNA sequence data and mass spectrometry data, we found evidence for several carbon fixation and assimilation strategies, including the Calvin-Benson-Bassham cycle, the reverse tricarboxylic acid cycle, the reductive acetyl coenzyme A (acetyl-CoA) pathway, and methylotrophy, in the microbial communities inhabiting the serpentinite-hosted aquifer. Our data also suggest that the microbial inhabitants of CROMO use products of the serpentinization process,...Continue Reading

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Citations

Apr 7, 2020·Environmental Microbiology·Mary C SabudaMatthew O Schrenk

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Datasets Mentioned

BETA
PRJNA410022
PRJNA410035
PRJNA410553
PRJNA410557
MTBLS1260

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

Ray Meta
CAMERA
CheckM
cutadapt
FastTree
Mummichog
KBase
HTSeq
XCMS
seq

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